Chemical structure of Beta-d-N4-Hydroxycytidine.
Beta-d-N4-Hydroxycytidine is supplied as a lyophilized powder. For a 15 mM stock, reconstitute 5 mg of powder in 1.28 ml of DMSO. Working concentrations and length of treatment can vary depending on the desired effect.
Store lyophilized at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, store at -20ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.
Molecular Weight: 259.2 g/mol
Molecular Formula: C9H13N3O6
Solubility: Soluble in DMSO at 25 mg/ml or water at 15 mg/ml with slight warming.
Beta-d-N4-Hydroxycytidine, also known as EIDD-1931, is a nucleic acid base analog and a specific AT to GC mutagen formed by the treatment of nucleoside cytidine with hydroxylamine (1). Beta-d-N4-Hydroxycytidine acts as a competitive substrate for viral encoded RNA polymerase and has demonstrated broad in vitro antiviral activity, including Chikungunya virus, Ebola, and Hepatitis C (2-4). Beta-d-N4-Hydroxycytidine demonstrated an IC50 value of 0.1 µM and an IC90 value of 6 µM in SARS-CoV infected Vero cells (5,6).
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