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15022
Carfilzomib
Activators & Inhibitors
Chemical Modulators

Carfilzomib #15022

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other Image 1 - Carfilzomib

Chemical structure of carfilzomib.

other Image 2 - Carfilzomib

Western blot analysis of extracts from NIH/3T3 cells, untreated (-) or treated with Carfilzomib (6 hr) at the indicated concentrations, using Ubiquitin Antibody #3933.

other Image 3 - Carfilzomib

Western blot analysis of extracts from NIH/3T3 cells, untreated (-) or treated with Carfilzomib (6 hr) at the indicated concentrations, using p53 (1C12) Mouse mAb #2524 (upper) or β-Actin (13E5) Rabbit mAb #4970 (lower).

Product Usage Information

Carfilzomib is supplied as a lyophilized powder. For a 5 mM stock, reconstitute the 1 mg of powder in 277.82 μl of DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 20-2000 nM for 4-48 hours.

Storage

Store lyophilized or in solution at -20ºC, desiccated. The chemical is stable for 24 months in lyophilized form. Once in solution, use within 1 week to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight 719.9 g/mol
Purity

>98%

Molecular Formula

C40H57N5O7

CAS 868540-17-4
Solubility Soluble in DMSO at 80mg/ml and EtOH at 25mg/ml.

Background

Carfilzomib, also known as PR-171, is a potent and irreversible epoxomycin-related proteasome inhibitor (1-4). It preferentially inhibits the chymotrypsin-like (CT-L) activity (low nanomolar IC50) of the 20S proteasome with greater than ten-fold selectivity over trypsin-like and caspase-like activities (1,2). CT-L inhibition with carfilzomib prevents degradation of short-lived misfolded and ubiquitinated proteins intended for proteasomal degradation, inducing cell cycle arrest and/or apoptosis in a variety of tumor cell lines (1-3). Carfilzomib has been shown to have greater antiproliferative activity against multiple myeloma (MM) cells than bortezomib and can overcome bortezomib-induced drug resistance (1). Synergistic interactions between carfilzomib and the histone deacetylase inhibitors vorinostat and entinostat have been observed (4). Proteasome inhibitors such as carfilzomib are important research tools for studying cellular degradation of the ubiquitin-proteasome pathway.

This product has applications to SARS-CoV-2 research into the mechanisms of the Novel Coronavirus, which has caused the COVID-19 pandemic.

  1. Kuhn, D.J. et al. (2007) Blood 110, 3281-90.
  2. Demo, S.D. et al. (2007) Cancer Res 67, 6383-91.
  3. Sacco, A. et al. (2011) Clin Cancer Res 17, 1753-64.
  4. Dasmahapatra, G. et al. (2011) Mol Cancer Ther 10, 1686-97.
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
To Purchase # 15022S
Product # Size Price
15022S
1 mg $ 75