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Santa Cruz discontinued a large number of its polyclonal products as a result of the USDA settlement that was made public May 19th 2016

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15022S 1 mg $69.00.0
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PhosphoSitePlus® Resource

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Western blot analysis of extracts from NIH/3T3 cells, untreated (-) or treated with Carfilzomib (6 hr) at the indicated concentrations, using Ubiquitin Antibody #3933.

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Western blot analysis of extracts from NIH/3T3 cells, untreated (-) or treated with Carfilzomib (6 hr) at the indicated concentrations, using p53 (1C12) Mouse mAb #2524 (upper) or β-Actin (13E5) Rabbit mAb #4970 (lower).

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Chemical structure of carfilzomib.

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Product Usage Information

Carfilzomib is supplied as a lyophilized powder. For a 5 mM stock, reconstitute the 1 mg of powder in 277.82 μl of DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 20-2000 nM for 4-48 hours.


Storage: Store lyophilized or in solution at -20ºC, desiccated. The chemical is stable for 24 months in lyophilized form. Once in solution, use within 1 week to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight:

719.9 g/mol


Purity:

>98%


Molecular Formula:

C40H57N5O7


Carfilzomib, also known as PR-171, is a potent and irreversible epoxomycin-related proteasome inhibitor (1-4). It preferentially inhibits the chymotrypsin-like (CT-L) activity (low nanomolar IC50) of the 20S proteasome with greater than ten-fold selectivity over trypsin-like and caspase-like activities (1,2). CT-L inhibition with carfilzomib prevents degradation of short-lived misfolded and ubiquitinated proteins intended for proteasomal degradation, inducing cell cycle arrest and/or apoptosis in a variety of tumor cell lines (1-3). Carfilzomib has been shown to have greater antiproliferative activity against multiple myeloma (MM) cells than bortezomib and can overcome bortezomib-induced drug resistance (1). Synergistic interactions between carfilzomib and the histone deacetylase inhibitors vorinostat and entinostat have been observed (4). Proteasome inhibitors such as carfilzomib are important research tools for studying cellular degradation of the ubiquitin-proteasome pathway.


1.  Kuhn, D.J. et al. (2007) Blood 110, 3281-90.

2.  Demo, S.D. et al. (2007) Cancer Res 67, 6383-91.

3.  Sacco, A. et al. (2011) Clin Cancer Res 17, 1753-64.

4.  Dasmahapatra, G. et al. (2011) Mol Cancer Ther 10, 1686-97.



For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

15022
Carfilzomib