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Cell Stimulation Cocktail (with Protein Transport Inhibitors) (500X)
Activators & Inhibitors
Chemical Modulators

Cell Stimulation Cocktail (with Protein Transport Inhibitors) (500X) #23318

Citations (0)
Flow cytometric analysis of live mouse splenocytes, untreated (left column) or treated with Cell Stimulation Cocktail (with Protein Transport Inhibitors) (500X; right column), using IFN-γ (XMG1.2) Rat mAb (APC Conjugate) #32793 (top row) or concentration-matched Rat Isotype Control (APC Conjugate) (bottom row), and co-stained with CD3 (17A2) Rat mAb (FITC Conjugate) #86603.

Product Usage Information

1. Prepare the cell suspension in the appropriate cell culture medium.

2. To obtain a 1X final concentration, add the Cell Stimulation Cocktail (with Protein Transport Inhibitors) (500X) directly to the cell suspension at 2 µL/mL.

3. Incubate the cells at 37°C in an atmosphere that contains CO2.

4. Harvest the cells for the immunoassay of interest.

Note: The concentration of cells and incubation time will vary depending on the desired results. This product is an ethanol solution and susceptible to evaporation. Keep tightly sealed when not in use.


Store at -20ºC tightly sealed to prevent evaporation and protected from light. This product is stable for at least 12 months when stored at -20ºC.

Product Description

Cell Stimulation Cocktail (with Protein Transport Inhibitors) (500X) is an ethanol solution that contains the phorbol ester, PMA (phorbol-12-myristate-13-acetate), the calcium ionophore, Ionomycin, and the protein transport inhibitors Brefeldin A and Monensin. When added in vitro to cell suspensions, the PMA and Ionomycin activate the cells, leading to increased cytokine production. The Brefeldin A and Monensin limit protein secretion, allowing for intracellular detection of the accumulated cytokines by downstream immunoassays.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not for Use in Diagnostic Procedures.
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To Purchase # 23318
Cat. # Size Qty. Price
100 µl