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Render Timestamp: 2024-10-11T09:53:55.317Z
Commit: 56767fe525c928647c8401233a175d0d607d385d
XML generation date: 2024-09-19 21:57:41.995
Product last modified at: 2024-09-19T22:12:48.024Z
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PDP - Template Name: Chemical Modulators
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Cell Stimulation Cocktail (without Protein Transport Inhibitors) (500X) #29255

    Product Information

    Product Usage Information

    1. Prepare the cell suspension in the appropriate cell culture medium.

    2. To obtain a 1X final concentration, add the Cell Stimulation Cocktail (without Protein Transport Inhibitors) (500X) directly to the cell suspension at 2 µL/mL.

    3. Incubate the cells at 37°C in an atmosphere that contains CO2.

    4. Harvest the cells for the immunoassay of interest.

    Note: The concentration of cells and incubation time will vary depending on the desired results. This product is an ethanol solution and susceptible to evaporation. Keep tightly sealed when not in use.

    Storage

    Store at -20ºC tightly sealed to prevent evaporation and protected from light. This product is stable for at least 12 months when stored at -20ºC.

    Product Description

    Cell Stimulation Cocktail (without Protein Transport Inhibitors) (500X) is an ethanol solution that contains the phorbol ester, PMA (phorbol-12-myristate-13-acetate) and the calcium ionophore, Ionomycin. When added in vitro to cell suspensions, the PMA and Ionomycin activate the cells, leading to increased cytokine production for detection by downstream immunoassays.
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    Cy and CyDye are registered trademarks of GE Healthcare.
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