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Chromomycin A3
Activators & Inhibitors
Cellular Dyes

Chromomycin A3 #79929

Citations (0)
Chemical structure of Chromomycin A3.

Product Usage Information

Chromomycin A3 is supplied as a lyophilized powder. For a 1 mM stock, reconstitute 5 mg of powder in 0.84 mL of DMSO. Working concentrations and length of treatment can vary depending on the desired effect.


Store lyophilized at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, store at -20ºC and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Fluorescent Properties

  • Excitation maximum = 458 nm
  • Emission maximum = 590 nm

Product Description

Molecular Weight 1183.3 g/mol
Purity >98%
Molecular Formula C57H82O26
CAS 7059-24-7
Solubility Soluble in DMSO at 20 mg/mL.


Chromomycin A3 is an antibiotic that binds to the GC-rich sequence of DNA in the presence of divalent cations, inhibiting DNA replication and transcription (1). It is also used as a fluorescent DNA stain, and is particularly useful in staining human sperm chromatin in fertility and protamine deficiency studies (2). An analog of Mithramycin A, Chromomycin A3 displays many of the same DNA-binding and transcriptional regulation properties. For example, Chromomycin A3 suppresses a similar set of specificity protein 1 (SP1)-related anti-apoptotic proteins as Mithramycin A, and effectively inhibits cell proliferation more strongly than Mithramycin A. In a comparative treatment of cervical cancer cells, Chromomycin A3 displayed an IC50 that was 4-10 times higher than the same cells treated with Mithramycin A. In several clinical studies, Chromomycin A3 exhibited anti-cancer effects in individuals with breast cancer, Hodgkin's disease, lung adenocarcinoma, melanoma, and rhabdomyosarcoma; however, there were also inconsistent treatment results and some harmful side effects (3).

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not for Use in Diagnostic Procedures.
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To Purchase # 79929
Cat. # Size Qty. Price
1 mg