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Cytochalasin D
Activators & Inhibitors
Chemical Modulators

Cytochalasin D #94946

Citations (0)
Chemical structure of Cytochalasin D.

Product Usage Information

Cytochalasin D is supplied as a lyophilized powder. For a 5 mM stock, reconstitute 1 mg of powder in 0.39 mL of DMSO. Working concentrations and length of treatment can vary depending on the desired effect.


Store lyophilized at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, store at -20ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight 507.6 g/mol
Purity >98%
Molecular Formula C30H37NO6
CAS 22144-77-0
Solubility Soluble in DMSO at 20 mg/mL or ethanol at 5 mg/mL.


The fungal-derived toxin Cytochalasin D is a potent inhibitor of actin polymerization, limiting elongation at both the barbed and pointed ends of the actin filament (1). While several related Cytochalasin toxins bind actin and alter polymerization, Cytochalasin D is the most specific inhibitor of actin microfilaments (2). The cytoskeleton takes part in signal transduction, protein transport, signal compartmentalization, and cell mobility. Depolymerization of the actin cytoskeleton by Cytochalasin D in mesenchymal stem cells results in localization of G-actin to the nucleus, which leads to mesenchymal stem cell differentiation and bone formation (3). Treatment of a pair of cancer cell lines with Cytochalasin D results in the inhibition of actin polymerization and decreased cell motility (4). Treatment of murine CT26 colorectal carcinoma cells with Cytochalasin D inhibited tumor growth through reduced cell proliferation, induction of cell apoptosis, and suppression of tumor angiogenesis (5).

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.
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To Purchase # 94946
Cat. # Size Qty. Price
1 mg