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XML generation date: 2024-09-20 06:15:26.449
Product last modified at: 2024-09-26T21:15:09.268Z
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PDP - Template Name: Chemical Modulators
PDP - Template ID: *******c501c72

DAPT #15020

    Product Information

    Product Usage Information

    DAPT is supplied as a lyophilized powder. For a 25 mM stock, reconstitute 5 mg in 462.43 μl DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but are typically used at 10-50 μM for 12-48 hr, respectively.

    Storage

    Store lyophilized or in solution at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

    Product Description

    Molecular Weight 432.5 g/mol
    Purity >95%
    Molecular Formula C23H26F2N2O4
    CAS 208255-80-5
    Solubility Soluble in DMSO at 15mg/ml and EtOH at 1mg/ml.

    Background

    DAPT is a potent γ-secretase inhibitor. Research studies have demonstrated that DAPT inhibits production of total β-amyloid peptide (Aβ) and Aβ42 in human primary neuronal cultures with an IC50 of 115 nM and 200 nM, respectively (1). DAPT-induced inhibition of Notch1 signaling prevents cleavage formation of the Notch1 intracellular domain (NICD), resulting in down regulation of target gene transcription (2). DAPT treatment has been shown to induce apoptosis in Jurkat (2) and lung squamous cell carcinoma (3) cell lines.
    For Research Use Only. Not For Use In Diagnostic Procedures.
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