Buy 3 and get the 4th FREE!* | Learn More >>
15020
DAPT
Activators & Inhibitors

DAPT #15020

Reviews ()
Citations (0)

Chemical structure of DAPT.

Western blot analysis of extracts from Jurkat cells, untreated (-) or treated with DAPT (24 hr) at the indicated concentrations, using Cleaved Notch1 (Val1744) (D3B8) Rabbit mAb #4147 (upper), Notch1 (D6F11) XP® Rabbit mAb #4380 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Product Usage Information

DAPT is supplied as a lyophilized powder. For a 25 mM stock, reconstitute 5 mg in 462.43 μl DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but are typically used at 10-50 μM for 12-48 hr, respectively.

Storage:

Store lyophilized or in solution at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight:

432.5 g/mol

Purity:

>95%

Molecular Formula:

C23H26F2N2O4

Background

DAPT is a potent γ-secretase inhibitor. Research studies have demonstrated that DAPT inhibits production of total β-amyloid peptide (Aβ) and Aβ42 in human primary neuronal cultures with an IC50 of 115 nM and 200 nM, respectively (1). DAPT-induced inhibition of Notch1 signaling prevents cleavage formation of the Notch1 intracellular domain (NICD), resulting in down regulation of target gene transcription (2). DAPT treatment has been shown to induce apoptosis in Jurkat (2) and lung squamous cell carcinoma (3) cell lines.

  1. Dovey, H.F. et al. (2001) J Neurochem 76, 173-81.
  2. Luo, X. et al. (2013) Cancer Cell Int 13, 34.
  3. Cao, H. et al. (2012) APMIS 120, 441-50.
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.

To Purchase # 15020S
Product # Size Price
15020S
5 mg $ 105