Dexamethasone #14776

Product Usage Information

Dexamethasone is supplied as a lyophilized powder. For a 10 mM stock, reconstitute the 5 mg in 1.27 ml DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 10-1,000 nM for 1-24 hr.

Storage

Store lyophilized or in solution at -20ºC, desiccated. Protect from light. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight:

392.5 g/mol

Purity:

>98%

Molecular Formula:

C₂₂H₂₉FO₅

Background

Dexamethasone is a synthetic glucocorticoid that binds to the human glucocorticoid receptor (GR). Treatment of cells with dexamethasone leads to nuclear translocation and activation of GR. This elicits anti-inflammatory, cellular proliferation, and metabolic responses via GR-mediated transcriptional activation or repression of target genes (1-4). Research studies show that dexamethasone inhibits dendritic cell differentiation and maturation (5). The inhibition of nitric oxide synthase by dexamethasone has also been observed (6).

1. Matthews, L. et al. (2009) Endocrinology 150, 75-86.
2. Cole, T.J. (2006) Biotechnol Annu Rev 12, 269-300.
3. Beck, I.M. et al. (2009) Endocr Rev 30, 830-82.
4. Matasić, R. et al. (1999) J Leukoc Biol 66, 909-14.
5. Moncada, S. and Palmer, R.M. (1991) Trends Pharmacol Sci 12, 130-1.
6. Wang, Z. et al. (2002) J. Biol. Chem. 277, 26573-80.