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Western blot analysis of extracts from HeLa cells, serum-starved overnight and untreated (-) or treated with Dexamethasone (1 hr) at the indicated concentrations, using Phospho-Glucocorticoid Receptor (Ser211) Antibody #4161 (upper) and Glucocorticoid Receptor (D6H2L) XP® Rabbit mAb #12041 (lower).Learn more about how we get our images
Western blot analysis of extracts from A549 cells, untreated (-) or treated with Dexamethasone (24 hr) at the indicated concentrations, using β-Arrestin 1 (D8O3J) Rabbit mAb #12697 (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).Learn more about how we get our images
Gallery: Dexamethasone #14776
Dexamethasone is supplied as a lyophilized powder. For a 10 mM stock, reconstitute the 5 mg in 1.27 ml DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 10-1,000 nM for 1-24 hr.Storage: Store lyophilized or in solution at -20ºC, desiccated. Protect from light. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.
Dexamethasone is a synthetic glucocorticoid that binds to the human glucocorticoid receptor (GR). Treatment of cells with dexamethasone leads to nuclear translocation and activation of GR. This elicits anti-inflammatory, cellular proliferation, and metabolic responses via GR-mediated transcriptional activation or repression of target genes (1-4). Research studies show that dexamethasone inhibits dendritic cell differentiation and maturation (5). The inhibition of nitric oxide synthase by dexamethasone has also been observed (6).
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. XP is a registered trademark of Cell Signaling Technology, Inc.