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9886
Docetaxel

Docetaxel #9886

Western Blotting

Western blot analysis of extracts from HT-29 cells treated with Docetaxel (100 ng/ml) for the indicated times using Cyclin B1 Antibody #4138 (upper) or Phospho-Aurora A (Thr288) (C39D8) Rabbit mAb #3079 (lower).

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Structure

Docetaxel is supplied as 1 mg powder. Store at or below -20ºC. Before use, dissolve powder in 1 ml DMSO to make a 1 mg/ml stock solution. The suggested working concentration is 100 ng/ml in tissue culture medium. Treat cells for 4-24 hours. Store solution at -20ºC.

Storage:

Store lyophilized or in solution at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Molecular Weight:

807.89 g/mol

Purity:

>99%

Molecular Formula:

C43H53NO14

Docetaxel belongs to the taxane family of antitumor and antileukemic agents (1). A microtubule-stabilizing agent, docetaxel acts as an inhibitor of cellular functions that require microtubule dynamics including cell division, and is now in use as a treatment for breast, prostate and lung cancer (2).

  1. Abal, M. et al. (2003) Curr Cancer Drug Targets 3, 193-203.
  2. Crown, J. and O'Leary, M. (2000) Lancet 355, 1176-8.
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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