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Render Timestamp: 2024-12-10T10:56:43.757Z
Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-09-20 06:23:32.822
Product last modified at: 2024-05-30T07:11:02.009Z
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PDP - Template Name: Chemical Modulators
PDP - Template ID: *******c501c72

E-64 #90267

    Product Information

    Product Usage Information

    E-64 is supplied as a lyophilized powder. For a 15 mM stock, reconstitute 5 mg of powder in 0.93 mL of DMSO. Working concentrations and length of treatment can vary depending on the desired effect.

    Storage

    Store lyophilized at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, store at -20ºC and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

    Product Description

    Molecular Weight 357.4 g/mol
    Purity >99%
    Molecular Formula C15H27N5O5
    CAS 66701-25-5
    Solubility Soluble in 1:1 mixture of water or ethanol at 20 mg/mL.

    Background

    The small-molecule E-64 is an anti-parasitic and anti-malarial agent that acts as an irreversible, non-selective inhibitor of cysteine proteases. E-64 binds and inhibits the cysteine proteases cathepsin K (IC50 = 1.4 nM), cathepsin S (IC50 = 4.1 nM), and cathepsin L (IC50 = 2.5 nM) in human osteoclast assays. E-64 also inhibits other proteases, including cathepsin B, cathepsin H, papain, and calpain (1,2). Exposure of filarial parasites to E-64 inhibited cathepsin B and resulted in decreased parasite motility and viability (3). E-64 effectively inhibited a SUMO-1-like cysteine protease from the African swine fever virus, resulting in impaired viral replication and a possible treatment for a viral disease that typically causes high mortality in domestic pigs (4). Inhibition of cathepsin B by E-64 in tumor cell lines reduced the invasive properties of the cells by 75% (5). E-64 treatment of ischemia-reperfusion rat kidneys blocked calpain activity and reduced several markers of renal dysfunction (6).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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