Interested in promotions? | Click here >>
Activators & Inhibitors
Chemical Modulators

Etoposide #2200

Reviews ()
Citations (0)
other Image 1 - Etoposide

Chemical structure of etoposide.

other Image 2 - Etoposide

Western blot analysis of extracts from Jurkat cells, untreated (-) or etoposide-treated (25 μM, overnight; +), using Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb #5625 (upper) or total PARP Antibody #9542 (lower).

Product Usage Information

Etoposide is supplied as a lyophilized powder. For a 50 mM stock, reconstitute the 5.9 mg in 200 µl DMSO. Working concentrations and length of treatments vary depending on the desired effect, but it is typically used at 5-50 µM for 4-24 hr. Soluble in DMSO at 100 mg/ml; very poorly soluble in ethanol and water with maximum solubility in water ~20-50 µM.


Store lyophilized or in solution at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight 588.56 g/mol


Molecular Formula


CAS 33419-42-0
Solubility Soluble in DMSO at 25mg/ml.


An anti-tumor agent that is commonly used as an apoptosis inducer, etoposide (VP-16) is a topoisomerase II inhibitor with an IC50 of 59.2 µM (1). Etoposide stabilizes a covalent topoisomerase II-cleaved DNA intermediate complex in the catalytic cycle of the enzyme, leading to genomic instability and cell death (2,3). This mechanism of action has been shown to delay progression of the cell cycle through the late S and early G2 phase (4,5).

  1. Terada, T. et al. (1993) J Med Chem 36, 1689-99.
  2. Baldwin, E.L. and Osheroff, N. (2005) Curr Med Chem Anticancer Agents 5, 363-72.
  3. Li, T.K. and Liu, L.F. (2001) Annu. Rev. Pharmacol. Toxicol. 41, 53-77.
  4. Dołega, A. (1998) Postepy Hig Med Dosw 52, 67-87.
  5. Smith, P.J. et al. (1994) Br J Cancer 70, 914-21.
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
To Purchase # 2200S
Product # Size Price
5.9 mg $ 108