The purity of recombinant hIL-21 was determined by SDS-PAGE of 1.5 µg reduced (+) and non-reduced (-) recombinant hIL-21 and staining overnight with Coomassie Blue.Learn more about how we get our images
Serial dilutions of Human IL-21 were added to B9 cells. Cell proliferation was assessed after 72 hours by measuring OD490.Learn more about how we get our images
Western blot analysis of extracts from Jurkat cells, untreated or treated with hIL-21 for 20 minutes, using Phospho-Stat3 (Tyr705) (D3A7) XP™ Rabbit mAb #9145 (upper) or Stat3 (124H6) Mouse mAb #9139 (lower).Learn more about how we get our images
Recombinant human IL-21 was expressed in E. coli and is supplied in a lyophilized form. A greater than 95% purity was determined by SDS-PAGE. Endotoxin levels are less than or equal to 1 EU / 1 μg hIL-21.
The bioactivity of recombinant hIL-21 was determined by its ability to induce IFN-γ production in B9 cells. The ED50 of each lot is between 25-50 ng/ml.
Working concentration of hIL-21 generally ranges from 0.1-10 ng/ml.
Recombinant human IL-21 is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/ml which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long term storage.
IL-21 is produced by Th17, T follicular helper (Tfh) and NKT cells (1-3). Targets include T cells, B cells, NK cells and dendritic cells (1-3,5). IL-21 induces proliferation and activation of NK cells, thereby up-regulating IFN-γ production and cytotoxic activity (1,3,4). IL-21 increases proliferation and survival of CD40-primed B cells (1,2) and appears to have a significant role in plasma cell differentiation (2). IL-21 binds to a complex consisting of IL-21Rα and the common γ chain, γc. IL-21 binding activates the Jak1/Jak3, Stat1, Stat3 and Stat5 pathway. IL-21 binding can also activate the MAP kinase and PI3K/Akt pathways.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. XP is a registered trademark of Cell Signaling Technology, Inc.
Explore pathways related to this product.