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45095
Human Interleukin-21 (hIL-21) Recombinant Protein
Cytokines & Growth Factors
Growth Factors and Cytokines

Human Interleukin-21 (hIL-21) Recombinant Protein #45095

Citations (0)
Serial dilutions of recombinant human IL-21 were added to B9 cells. Cell proliferation was assessed after 72 hours by measuring OD490.
The purity of recombinant hIL-21 was determined by SDS-PAGE of 1.5 µg reduced (+) and non-reduced (-) recombinant hIL-21 and staining overnight with Coomassie Blue.
Western blot analysis of extracts from Jurkat cells, untreated or treated with recombinant hIL-21 for 20 minutes, using Phospho-Stat3 (Tyr705) (D3A7) XP Rabbit mAb #9145 (upper) or Stat3 (124H6) Mouse mAb #9139 (lower).

Product Usage Information

Working concentration of recombinant hIL-21 generally ranges from 0.1-10 ng/ml.

Storage

Recombinant human IL-21 is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/ml which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long term storage.

Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.

Product Description

MW (kDa) 16
Purity A greater than 95% purity was determined by SDS-PAGE.
Endotoxin Less than or equal to 1 EU / 1 μg hIL-21.
Activity The bioactivity of recombinant hIL-21 was determined by its ability to induce IFN-γ production in B9 cells. The ED50 of each lot is between 25-50 ng/ml.

Source / Purification

Recombinant human IL-21 was expressed in E. coli and is supplied in a lyophilized form.

Background

IL-21 is produced by Th17, T follicular helper (Tfh) and NKT cells (1-3). Targets include T cells, B cells, NK cells and dendritic cells (1-3,5). IL-21 induces proliferation and activation of NK cells, thereby up-regulating IFN-γ production and cytotoxic activity (1,3,4). IL-21 increases proliferation and survival of CD40-primed B cells (1,2) and appears to have a significant role in plasma cell differentiation (2). IL-21 binds to a complex consisting of IL-21Rα and the common γ chain, γc. IL-21 binding activates the Jak1/Jak3, Stat1, Stat3 and Stat5 pathway. IL-21 binding can also activate the MAP kinase and PI3K/Akt pathways.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not for Use in Diagnostic Procedures.
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To Purchase # 45095
Cat. # Size Qty. Price
45095S
10 µg