News from the Bench

Discover what’s going on at CST, receive our latest application notes and tips, read our science features, and learn about our products.

Subscribe

Dec 31st Santa Cruz will discontinue a
large number of polyclonal products as
a result of the USDA settlement that was
made public May 19th.

Find CST Equivalent

Questions?

Find answers on our FAQs page.

ANSWERS  

PhosphoSitePlus® Resource

  • Additional protein information
  • Analytical tools

LEARN MORE

Western blot analysis of extracts from C2C12 cells, untreated (-) or treated with Forskolin #3828 (10 μM, 30 min; +) with or without IBMX pretreatment (4 hr) at the indicated concentrations, using Phospho-VASP (Ser157) Antibody #3111 (upper), Phospho-VASP (Ser239) Antibody #3114 (middle), or VASP (9A2) Rabbit mAb #3132 (lower).

Learn more about how we get our images

Chemical structure of IBMX.

Learn more about how we get our images
Image

Product Usage Information

IBMX is supplied as a lyophilized powder. For a 250 mM stock, reconstitute the 50 mg in 899.7 µl DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 10-1,000 µM for 15 min-4 hr.


Storage: Store lyophilized or in solution at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight:

222.30 g/mol


Purity:

>98%


Molecular Formula:

C10H14N4O2


3-isobutyl-1-methylxanthine (IBMX) is a nonselective inhibitor of cAMP and cGMP phosphodiesterases (PDEs). Research studies show that IBMX inhibits many members of the PDE family, with the exceptions of PDE8A, PDE8B, and PDE9 (1-4). Treatment of cells with IBMX promotes accumulation of cAMP and cGMP, which leads to activation of cyclic-nucleotide-regulated protein kinases (5,6). Additional research indicates that IBMX can promote neuronal progenitor cell maturation in vitro (7).


1.  Fawcett, L. et al. (2000) Proc Natl Acad Sci U S A 97, 3702-7.

2.  Fisher, D.A. et al. (1998) Biochem Biophys Res Commun 246, 570-7.

3.  Hayashi, M. et al. (1998) Biochem Biophys Res Commun 250, 751-6.

4.  Soderling, S.H. et al. (1998) J Biol Chem 273, 15553-8.

5.  Chanoit, G. et al. (2011) Cardiovasc Drugs Ther 25, 299-306.

6.  Zhang, R. et al. (2011) PLoS One 6, e20780.

7.  Lepski, G. et al. (2013) Front Cell Neurosci 7, 155.



For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

13630
IBMX