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9995
Ionomycin, Calcium Salt
Activators & Inhibitors
Chemical Modulators

Ionomycin, Calcium Salt #9995

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Ionomycin, Calcium Salt: Image 1

Confocal immunofluorescent analysis of MCF7 cells, untreated (left) or treated with Ionomycin (1 μM, 1 hr; right), using NFAT1 (D43B1) XP® Rabbit mAb #5861 (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Ionomycin, Calcium Salt: Image 2

Structure of Ionomycin

Product Usage Information

Ionomycin, calcium salt is supplied as 2.5 mg powder. Store at or below -20ºC. Before use, dissolve powder in 1.1 ml DMSO to make a 3 mM ionomycin stock solution. For working concentrations of 1 μM-3 μM, dilute DMSO stock 1:3000 to 1:1000. Treat cells with the desired concentration for intended time.

Storage

Store lyophilized or in solution at -20ºC, desiccated. Protect from light. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight 747.08 g/mol
Purity

>99%

Molecular Formula

C41H70O9 • Ca2+

CAS 56092-82-1
Solubility Soluble in DMSO at 1.6mg/ml and EtOH at 20mg/ml.

Background

Ionomycin is a potent and selective calcium ionophore agent (1,2). The molecule acts as a motile Ca2+ carrier and enhances Ca2+ influx by direct stimulation of store-regulated cation entry across biological membranes (3). At the micromolar level, ionomycin can activate Ca2+/Calmodulin dependent kinase and phosphatase to stimulate gene expression (4). In human T cells, ionomycin induces hydrolysis of phosphoinositides and activates PKC to mediate T cell activation (5). Ionomycin treatment of human B cells induces the activation of calcium-dependent endonuclease and results in apoptosis (6). Ionomycin treatment of bovine aortic endothelial cells (BAECs) induces rapid dephosphorylation of eNOS at Thr495 and mediates eNOS activation (7).

  1. Liu, C. and Hermann, T.E. (1978) J Biol Chem 253, 5892-4.
  2. Kauffman, R.F. et al. (1980) J Biol Chem 255, 2735-9.
  3. Morgan, A.J. and Jacob, R. (1994) Biochem J 300 ( Pt 3), 665-72.
  4. Lobo, F.M. et al. (1999) J Immunol 162, 2057-63.
  5. Chatila, T. et al. (1989) J Immunol 143, 1283-9.
  6. Aagaard-Tillery, K.M. and Jelinek, D.F. (1995) J Immunol 155, 3297-307.
  7. Lin, M.I. et al. (2003) J Biol Chem 278, 44719-26.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
To Purchase # 9995S
Product # Size Price
9995S
2.5 mg $ 228