KU-55933 #83346

Product Usage Information

KU-55933 is supplied as a lyophilized powder. For a 10 mM stock, reconstitute 5 mg of powder in 1.26 ml of DMSO. Working concentrations and length of treatment can vary depending on the desired effect.

Storage

Store lyophilized at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, store at -20ºC and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight: 395.5 g/mol

Purity: >98%

Molecular Formula: C₂₁H₁₇NO₃S₂

CAS: 587871-26-9

Solubility: Soluble in DMSO at 40 mg/ml or ethanol at 20 mg/ml.

Background

KU-55933 is a potent and selective ATP-competitive inhibitor of ATM kinase (IC₅₀ = 13 nM and K_i = 2.2 nM). This small molecule has been shown to sensitize cancer cells to ionizing radiation and chemotherapeutic agents (1). Treating senescent cancer cells (MCF7, A549, and HCT 116) with KU-55933 results in decreased viability and cell death (2). KU-55933 has neuroprotective capabilities by preventing damage caused by oxidative stress (3).

1. Hickson, I. et al. (2004) Cancer Res 64, 9152-9.
2. Crescenzi, E. et al. (2008) Clin Cancer Res 14, 1877-87.
3. Chwastek, J. et al. (2017) Int J Biochem Cell Biol 87, 38-53.