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51854
Mitomycin C
Activators & Inhibitors
Chemical Modulators

Mitomycin C #51854

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Mitomycin C: Image 1
Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Mitomycin C (2 μg/mL, 24 hr; +), using FANCD2 (D5L5X) Rabbit mAb #16323 (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). DNA damage caused by Mitomycin C induces monoubiquitination of FANCD2, altering its electrophoretic mobility and increasing its apparent molecular weight (5).
Mitomycin C: Image 2
Chemical structure of Mitomycin C.

Product Usage Information

Mitomycin C is supplied as a lyophilized powder. For a 15 mM stock, reconstitute 5 mg of powder in 997 μl of DMSO. Working concentrations and length of treatment can vary depending on the desired effect.

Storage

Store lyophilized at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, store at -20ºC and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight 334.3 g/mol
Purity >98%
Molecular Formula C15H18N4O5
CAS 50-07-7
Solubility Soluble in DMSO at 15 mg/ml or water at 1 mg/ml with slight warming.

Background

Mitomycin C, also known as MMC, is an antitumor antibiotic isolated from Streptomyces caespitosus (1). When activated, this alkylating agent crosslinks double stranded DNA resulting in DNA damage (2). Mitomycin C has been found to have lethal effects on HeLa cells during the G1 phase of the cell cycle (3). DNA damage caused by Mitomycin C can lead to increased levels of p53 that can result in cell cycle arrest or apoptosis (4).
  1. HATA, T. and SUGAWARA, R. (1956) <cite>J Antibiot (Tokyo)</cite> 9, 147-51.
  2. Tomasz, M. (1995) <cite>Chem Biol</cite> 2, 575-9.
  3. Djordjevic, B. and Kim, J.H. (1968) <cite>J Cell Biol</cite> 38, 477-82.
  4. Tishler, R.B. et al. (1993) <cite>Cancer Res</cite> 53, 2212-6.
  5. Garcia-Higuera, I. et al. (2001) <cite>Mol Cell</cite> 7, 249-62.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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To Purchase # 51854S
Product # Size Price
51854S
5 mg $ 75