Chemical structure of Nafamostat Mesylate.
Nafamostat Mesylate is supplied as a lyophilized powder. For a 15 mM stock, reconstitute 10 mg of powder in 1.23 ml of DMSO. Working concentrations and length of treatment can vary depending on the desired effect.
Store lyophilized at room temperature, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, store at 4ºC and use within 1 month or at -80ºC and use within 6 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.
Molecular Weight: 539.6 g/mol
Molecular Formula: C19H17N5O2 • 2CH3SO3H
Solubility: Soluble in DMSO at 50 mg/ml or water at 50 mg/ml.
Nafamostat Mesylate, also known as FUT-175, is a potent and reversible, broad-spectrum serine protease inhibitor of trypsin (Ki = 15 nM), tryptase (Ki = 95.3 pM), and other additional proteases (1). This small compound has been identified as a key player in the disruption of the inflammatory response via inhibition of the complement system and coagulation cascade (2). Its wide range of inhibitory activity displays anti-cancer and anti-viral properties and has shown effective inhibition of MERS-CoV by preventing membrane fusion, making it a promising therapeutic candidate against the 2019-nCoV infection (EC50 = 22.50 μM) (3,4).
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