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17802
Necrostatin-1s
Activators & Inhibitors

Necrostatin-1s #17802

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Western blot analysis of HT-29 cells, untreated (-) or treated with combinations of the following treatments as indicated: Z-VAD(OMe)-FMK #60332 (Z-VAD, 20 μM, added 30 min prior to other compounds; +), human TNF-α #8902 (hTNF-α, 20 ng/ml, 7 hr; +), SM-164 (100 nM, 7 hr; +), and necrostatin-1s (Nec-1s, indicated concentrations, 7 hr), using Phospho-RIP (Ser166) (D1L3S) Rabbit mAb #65746 (upper), RIP (D94C12) XP® Rabbit mAb #3493 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Chemical structure of Necrostatin-1s.

Product Usage Information

Necrostatin-1s is supplied as a lyophilized powder. For a 20 mM stock, reconstitute 5 mg of powder in 900 μl of DMSO. Working concentrations and length of treatment can vary depending on the desired effect.

Storage:

Store lyophilized at room temperature, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, store at -20ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight: 277.7 g/mol

Purity: >98%

Molecular Formula: C13H12CIN3O2

CAS: 852391-15-2

Solubility: Soluble in DMSO at 25 mg/ml.

Background

Necrostatin-1s, also known as Nec-1s or 7-Cl-O-Nec-1, is a potent and selective inhibitor of necroptosis. This analog of Necrostatin-1 (Nec-1) has been shown to be a specific receptor-interacting protein kinase (RIPK) 1 inhibitor that is metabolically more stable than the other variants (1). Research studies have found that Nec-1s is more selective than Nec-1 and Nec-1i (inactive variant) due to the fact that both molecules have an inhibitory affect on indoleamine 2,3-dioxygenase (IDO), a potent immunosuppressive molecule (2). Evidence also suggests that Nec-1s is around two-times more effective at RIPK1 inhibition than Nec-1 (IC50 = 210 nM vs. 494 nM) (1).

  1. Mikuš, P. et al. (2018) Molecules 23, .
  2. Takahashi, N. et al. (2012) Cell Death Dis 3, e437.
For Research Use Only. Not For Use In Diagnostic Procedures.

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To Purchase # 17802S
Product # Size Price
17802S
5 mg $ 68