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66419
Nigericin (sodium salt)
Activators & Inhibitors
Chemical Modulators

Nigericin (sodium salt) #66419

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Nigericin (sodium salt): Image 1
Western blot analysis of extracts from mouse primary bone marrow-derived macrophage (BMDM) cells, untreated (-), or treated (+) with Lipopolysaccharides (LPS) #14011 (50 ng/ml, 4 hr) and Nigericin (sodium salt) (45 min) at the indicated concentrations. Western blot analysis was performed using IL-1β (D3H1Z) Rabbit mAb (Mouse Specific) #12507 (upper), Cleaved-IL-1β (Asp117) Antibody (Mouse Specific) #52718 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Nigericin (sodium salt): Image 2
Confocal immunofluorescent analysis of mouse primary bone marrow-derived macrophages (BMDMs), untreated (left) or treated with Lipopolysaccharides (LPS) #14011 (50 ng/ml, 4 hr) followed by Nigericin (sodium salt) (15 μM, 45 min; right), using ASC/TMS1 (D2W8U) Rabbit mAb (Mouse Specific) #67824 (green). Blue = Hoechst 33342 #4082 (fluorescent DNA dye). Note the translocation of ASC to inflammasomes following stimulation with LPS and Nigericin (sodium salt).
Nigericin (sodium salt): Image 3
Chemical structure of Nigericin (sodium salt).

Product Usage Information

Nigericin (sodium salt) is supplied as a lyophilized powder. For a 10 mM stock, reconstitute 5 mg of powder in 669 μl of DMSO. Working concentrations and length of treatment can vary depending on the desired effect.

Storage

Store lyophilized at 4ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, store at -20ºC and use within 2 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight 747 g/mol
Purity >98%
Molecular Formula C40H67O11 • Na
CAS 28643-80-3
Solubility Soluble in DMSO at 50 mg/ml and EtOH at 20 mg/ml.

Background

Nigericin (sodium salt) is an electroneutral K+/H+ ionophore derived from S. hygroscopicus. This antibiotic is lipophilic and selectively causes efflux of potassium across mitochondrial membranes (1,2). The ion exchange can lead to disruption of membrane potential, changes in pH, and cell death making the compound useful for studying areas of interests such as apoptosis, mitosis, and the immune response (3).
  1. Fruth, I.A. and Arrizabalaga, G. (2007) Int J Parasitol 37, 1559-67.
  2. Kucejova, B. et al. (2005) Genetics 171, 517-26.
  3. Lim, J. et al. (2012) J Biol Chem 287, 23271-82.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
To Purchase # 66419
Cat. # Size Qty. Price
66419S
5 mg $ 87