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9807
Paclitaxel

Paclitaxel #9807

Western Blotting

Western blot analysis of extracts from HT29 cells, either untreated or treated with indicated concentration of paclitaxel using Phospho-Cdc25C (Thr48) Antibody #9527 (upper) or control Cdc25C (5H9) Rabbit mAb Antibody #4688 (lower).

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Structure

Structure of Paclitaxel

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Paclitaxel is supplied as 1 mg powder. Store at or below -20ºC. Before use, dissolve powder in 1.15 ml DMSO to make a 1 mM paclitaxel stock solution. For working concentrations of 100 nM-1000 nM, dilute DMSO stock 1:10,000 to 1:1000. Treat cells with the desired concentration for 6-48 hours.

Storage:

Store lyophilized or in solution at -20ºC, desiccated. Protect from light. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Molecular Weight:

853.92 g/mol

Purity:

>99.5%

Molecular Formula:

C47H51NO14

Paclitaxel belongs to the taxane family of antitumor and antileukemic agents (3). By binding to β-tubulin and promoting the assembly of microtubules, paclitaxel prevents microtubual depolymerization and blocks normal cell division (1-3). The microtubule dysfunction induced by paclitaxel results in aberrant cell mitosis and sometimes apoptosis. The IC50 of paclitaxel-induced mitotic block is 4 nM (4).

  1. Rowinsky, E.K. et al. (1990) J. Natl. Cancer Inst. 82, 1247-1259.
  2. Jordan, A. et al. (1998) Med. Res. Rev. 18, 259-296.
  3. Abal, M. et al. (2003) Curr Cancer Drug Targets 3, 193-203.
  4. Long, B.H. and Fairchild, C.R. (1994) Cancer Res. 54, 4355-4361.
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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