Dropping with the temps: Cool deals on CST mAbs | Learn More >>
Activators & Inhibitors

PX-866 #13055

Western Blotting

Western blot analysis of extracts from Jurkat cells, untreated (-) or treated with PX-866 (1 μM; +) or Wortmannin #9951 (1 μM; +) for the indicated times, using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 (upper) and Akt (pan) (C67E7) Rabbit mAb #4691 (lower).

Learn more about how we get our images

PX-866 is supplied as a lyophilized powder. For a 10mM stock, reconstitute the 1 mg in 190.26 μl DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 10 nM-1000 nM for 1-24 hours.


Store lyophilized or in solution at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Molecular Weight:

525.59 g/mol



Molecular Formula:



Solubility: Soluble in DMSO and ethanol at 200 mg/ml. Very poorly soluble in water.

PX-866 is a potent derivative of wortmannin that irreversibly inhibits PI3 kinase (1). Research studies demonstrate that PX-866 elicits a more sustained inhibition of PI3K than wortmannin. While both chemicals inhibit Akt phosphorylation similarly at short time periods (1-2 hr), PX-866 also demonstrates inhibitory effects for extended time periods (24 hr) at lower doses than wortmannin. This difference may be attributed to wortmannin’s short half-life of 10 minutes and the reduced cytotoxicity of PX-866 (2). PX-866 inhibits the PI3K pathway through down regulation of cyclin D1 protein, which is responsible for regulating cell cycle progression and autophagy induction (3). Additional research shows that PX-866 inhibits cell migration in cancer cell lines (2).

  1. Ihle, N.T. et al. (2004) Mol Cancer Ther 3, 763-72.
  2. Howes, A.L. et al. (2007) Mol Cancer Ther 6, 2505-14.
  3. Koul, D. et al. (2010) Neuro Oncol 12, 559-69.
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.

News from the Bench

Discover what’s going on at CST, receive our latest application notes and tips, read our science features, and learn about our products.


To Purchase # 13055S

View sizes
Product # Size Price
1 mg $ 73.0