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12758
Thapsigargin
Activators & Inhibitors
Chemical Modulators

Thapsigargin #12758

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Thapsigargin: Image 1

Chemical structure of Thapsigargin.

Thapsigargin: Image 2

Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Thapsigargin (2 nM, 16 hr; +), using OS-9 (D8P4G) Rabbit mAb #12497 (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).

Thapsigargin: Image 3

Western blot analysis of extracts from C2C12 cells, untreated (-) or treated with Thapsigargin (300 nM, 30 min; +), using Phospho-eIF2α (Ser51) (D9G8) XP® Rabbit mAb #3398 (upper) and eIF2α Antibody #9722 (lower).

Product Usage Information

Thapsigargin is supplied as a lyophilized powder. For a 1.25 mM stock, reconstitute the 1 mg in 1.23 ml DMSO. Working concentrations and length of treatments vary depending on the desired effect, but it is typically used at 2-2000 nM for 0.5-24 hours.

Storage

Store lyophilized or in solution at -20ºC, dessicated. Protect from light. In lyophilized form, the chemical is stable for 24 months. Once reconstituted, chemical is stable for 1 week when stored at -20C. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight 650.8 g/mol
Purity

>98%

Molecular Formula

C34H50O12

CAS 67526-95-8
Solubility Soluble in DMSO at 65mg/ml and EtOH at 20mg/ml.

Background

Thapsigargin is a cell-permeable sesquiterpene lactone derived from the plant Thapsia garganica that acts as a tumor promoter in mammalian cells (1,2). Studies show that thapsagargin causes a rapid increase in cytosolic Ca2+ concentrations via discharge of intracellular Ca2+ stores. Research indicates that this increase in cytosolic calcium results from the specific inhibition of endoplasmic reticulum Ca2+-ATPases (IC50 = ~30 nM), and does not involve the hydrolysis of inositol phospholipids or protein kinase C (1,2). This disruption of calcium homeostasis is widely used in research studies to induce ER stress. Conflicting information regarding the role of thapsigargin in autophagy has been reported, but recent evidence points to thapsigargin inhibiting autophagy by blocking autophagosome fusion with lysosomes (3-5).

  1. Jackson, T.R. et al. (1988) Biochem J 253, 81-6.
  2. Thastrup, O. et al. (1990) Proc Natl Acad Sci U S A 87, 2466-70.
  3. Ding, W.X. et al. (2007) J Biol Chem 282, 4702-10.
  4. Grotemeier, A. et al. (2010) Cell Signal 22, 914-25.
  5. Ganley, I.G. et al. (2011) Mol Cell 42, 731-43.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
To Purchase # 12758S
Product # Size Price
12758S
1 mg $ 130