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XML generation date: 2024-09-20 06:14:29.336
Product last modified at: 2024-09-19T22:13:07.928Z
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PDP - Template Name: Chemical Modulators
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Thapsigargin #12758

    Product Information

    Product Usage Information

    Thapsigargin is supplied as a lyophilized powder. For a 1.25 mM stock, reconstitute the 1 mg in 1.23 ml DMSO. Working concentrations and length of treatments vary depending on the desired effect, but it is typically used at 2-2000 nM for 0.5-24 hours.

    Storage

    Store lyophilized or in solution at -20ºC, dessicated. Protect from light. In lyophilized form, the chemical is stable for 24 months. Once reconstituted, chemical is stable for 1 week when stored at -20C. Aliquot to avoid multiple freeze/thaw cycles.

    Product Description

    Molecular Weight 650.8 g/mol
    Purity >98%
    Molecular Formula C34H50O12
    CAS 67526-95-8
    Solubility Soluble in DMSO at 65mg/ml and EtOH at 20mg/ml.

    Background

    Thapsigargin is a cell-permeable sesquiterpene lactone derived from the plant Thapsia garganica that acts as a tumor promoter in mammalian cells (1,2). Studies show that thapsagargin causes a rapid increase in cytosolic Ca2+ concentrations via discharge of intracellular Ca2+ stores. Research indicates that this increase in cytosolic calcium results from the specific inhibition of endoplasmic reticulum Ca2+-ATPases (IC50 = ~30 nM), and does not involve the hydrolysis of inositol phospholipids or protein kinase C (1,2). This disruption of calcium homeostasis is widely used in research studies to induce ER stress. Conflicting information regarding the role of thapsigargin in autophagy has been reported, but recent evidence points to thapsigargin inhibiting autophagy by blocking autophagosome fusion with lysosomes (3-5).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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