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14379
Torin 1
Activators & Inhibitors
Chemical Modulators

Torin 1 #14379

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Torin 1: Image 1
Western blot analysis of extracts from MEF cells, untreated (-) or treated with Torin1 (1 hr) at the indicated concentrations, using Phospho-p70 S6 Kinase (Thr389) (108D2) Rabbit mAb #9234, p70 S6 Kinase (49D7) Rabbit mAb #2708, Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb #4858, S6 Ribosomal Protein (5G10) Rabbit mAb #2217, Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb #2855, or 4E-BP1 Antibody #9452.

Product Usage Information

Torin 1 is supplied as a lyophilized powder. For a 1 mM stock, reconstitute the 5 mg in 8.23 ml DMSO. First add 1 ml DMSO to the tube containing the chemical, vortex, and dispense into a new, larger tube. Repeat this action two or three more times to transfer any residual material. Add additional DMSO to the new tube to bring the volume up to 8.23 ml. Heating to 37ºC and/or additional vortexing may be required.

Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 10-1,000 nM for 1-24 hr.

Storage

Store lyophilized or in solution at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight 607.62 g/mol
Purity >98%
Molecular Formula C35H28F3N5O2
CAS 1222998-36-8
Solubility Soluble in DMSO at 2mg/ml.

Background

Torin 1 is a potent and selective ATP-competitive mTOR inhibitor (1,2). Researchers have shown that Torin 1 inhibits mTORC1 and mTORC2 in cell-free kinase assays with IC50 values of 2 nM and 10 nM, respectively (1,2). Torin 1 displays nearly 1000-fold selectivity for mTOR over PI3 Kinase (IC50 ~1.8 μM), 200-fold selectivity over ATM, DNA-PK, and hVps34, and does not significantly inhibit a panel of 353 serine/threonine, tyrosine, and lipid kinases at 10 μM. Torin 1 has also been shown to cause cell cycle arrest via a rapamycin-resistant mechanism independent of mTORC2 (1). Torin 1 treatment of cells leads to the dephosphorylation of mTOR downstream targets including p70 S6 kinase, S6 Ribosomal Protein, and 4E-BP1 (1-4). Activation and nuclear translocation of TFEB (EC50 = ~148 nM) by Torin 1 inhibition of mTORC1 has also been observed (5).
  1. Thoreen, C.C. et al. (2009) J Biol Chem 284, 8023-32.
  2. Liu, Q. et al. (2010) J Med Chem 53, 7146-55.
  3. Hong, S.K. et al. (2013) Int J Oncol 43, 2031-8.
  4. Peterson, T.R. et al. (2011) Cell 146, 408-20.
  5. Settembre, C. et al. (2012) EMBO J 31, 1095-108.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
To Purchase # 14379S
Product # Size Price
14379S
5 mg $ 155