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- Analytical tools
TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174
Western blot analysis of extracts from serum-starved 293 cells, untreated (lane 1), stimulated with TPA treatment (lanes 2 & 3), and treated with CIP and λ phosphatase (lane 3), using Phospho-PKC (pan) (βII Ser660) Antibody #9371 or PKCα Antibody #2056.Learn more about how we get our images
Gallery: TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174
TPA is supplied as a 1 mg powder. Store at -20C. Before use, dissolve powder in 1 ml DMSO to yield a 1.6 mM stock solution. For working concentration of 200 μM, add 125 μl stock solution to 875 μl DMSO. Treat cells with 200 nM TPA for 20-30 minutes.
TPA is soluble in DMSO and ethanol.Storage: Store lyophilized or in solution at -20ºC, desiccated. Protect from light. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.
TPA (12-O-tetradecanoylphorbol-13-acetate) / PMA (phorbol-12-myristate-13-acetate) is the most commonly used phorbol ester. It binds and activates protein kinase C, causing a wide range of effects in cells and tissues (1,2). TPA has been demonstrated to be a potent tumor promoter in mouse skin (3). Conversely, it has been shown to be an effective cancer therapeutic agent in myelocytic leukemia patients, and has been indicated as a potential colorectal cancer therapeutic (4-5). TPA has also been shown to increase white blood cell and neutrophil counts in solid tumor cancer patients (6).
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.