Chemical structure of Triptolide.
Triptolide is supplied as a lyophilized powder. For a 10 mM stock, reconstitute 5 mg of powder in 1.38 ml of DMSO. Working concentrations and length of treatment can vary depending on the desired effect.
Store lyophilized at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, store at -20ºC and use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.
Molecular Weight: 360.4 g/mol
Molecular Formula: C20H24O6
Solubility: Soluble in DMSO at 7 mg/ml.
Triptolide is an immunosuppressive and anti-inflammatory compound produced by the thunder god vine, Tripterygium wilfordii Hook F (1). Covalent binding of Triptolide to human XPB/ERCC3 inhibits its DNA-dependent ATPase activity and prevents nucleotide excision repair by the TFIIH complex (2). Triptolide has proven to be an effective synergistic therapy for inhibition of pancreatic cancer cell growth, with IC50 values of 0.01 µM and 0.02 µM in Capan-1 and Capan-2 adenocarcinoma cells, respectively (3,4). In addition to its anti-tumor activity, Triptolide has shown antiviral efficacy in HIV-1 and dengue virus via inhibition of Tat protein and COX-2, respectively (5,6).
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