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12819
Tunicamycin
Activators & Inhibitors

Tunicamycin #12819

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Chemical structure of Tunicamycin.

Western blot analysis of C2C12 cell extracts, untreated (-) or treated with Tunicamycin (2 μg/ml, 8 hr; +), using CHOP (D46F1) Rabbit mAb #5554 (upper) or β-Actin Antibody #4967 (lower).

Product Usage Information

Tunicamycin is supplied as a lyophilized powder. For a 5 mg/ml stock, reconstitute the 5 mg in 1 ml DMSO. Working concentrations and length of treatments vary depending on the desired effect, but it is typically used at 0.1-10 μg/ml for 0.5-20 hours.

Solubility: Soluble in DMSO, DMF or Pyridine.

Storage:

Store lyophilized or in solution at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight:

840.1 g/mol

Purity:

>98%

Molecular Formula:

Homolog A: C37H60N4O16

Homolog B: C38H62N4O16

Homolog C: C39H64N4O16

Homolog D: C40H66N4O16

Background

Isolated from Streptomyces lysosuperificus, tunicamycin is a nucleoside antibiotic that inhibits N-linked glycosylation in glycoprotein synthesis. Composed of tunicamycins A, B, C and D, the compound competitively blocks the transfer of N-acetylglucosamine-1-phosphate (GlcNAc-1-P) from UDP-GlcNAc to dolichol-P (1,2). By preventing glycoprotein synthesis, tunicamycin inhibits the formation of the “viral coat” known as the tunica or capsid in both RNA and DNA viruses, and thus exhibits antiviral properties (3). Research studies have shown that tunicamycin will arrest cells in G1 phase, preventing them from entering S-phase and increasing the expression of PERK (4,5). Following N-linked glycosylation inhibition, tunicamycin induces autophagy in response to ER stress, ultimately upregulating CHOP and BIP (6,7).

  1. Takatsuki, A. et al. (1971) J Antibiot (Tokyo) 24, 215-23.
  2. Harding, H.P. et al. (1999) Nature 397, 271-274.
  3. Kaushal, G.P. and Elbein, A.D. (1986) Plant Physiol 82, 748-52.
  4. Hirano, T. et al. (1982) J Biochem 92, 765-73.
  5. Brewer, J.W. and Diehl, J.A. (2000) Proc Natl Acad Sci U S A 97, 12625-30.
  6. Ding, W.X. et al. (2007) J Biol Chem 282, 4702-10.
  7. Zinszner, H. et al. (1998) Genes Dev 12, 982-95.
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

To Purchase # 12819S
Product # Size Price
12819S
5 mg $ 115