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12819
Tunicamycin
Activators & Inhibitors
Chemical Modulators

Tunicamycin #12819

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Tunicamycin: Image 1

Chemical structure of Tunicamycin.

Tunicamycin: Image 2

Western blot analysis of C2C12 cell extracts, untreated (-) or treated with Tunicamycin (2 μg/ml, 8 hr; +), using CHOP (D46F1) Rabbit mAb #5554 (upper) or β-Actin Antibody #4967 (lower).

Product Usage Information

Tunicamycin is supplied as a lyophilized powder. For a 5 mg/ml stock, reconstitute the 5 mg in 1 ml DMSO. Working concentrations and length of treatments vary depending on the desired effect, but it is typically used at 0.1-10 μg/ml for 0.5-20 hours.

Solubility: Soluble in DMSO, DMF or Pyridine.

Storage

Store lyophilized or in solution at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight 830.9 g/mol
Purity

>98%

Molecular Formula

Homolog A: C37H60N4O16

Homolog B: C38H62N4O16

Homolog C: C39H64N4O16

Homolog D: C40H66N4O16

CAS 11089-65-9
Solubility Soluble in DMSO at 40mg/ml and EtOH at 5mg/ml.

Background

Isolated from Streptomyces lysosuperificus, tunicamycin is a nucleoside antibiotic that inhibits N-linked glycosylation in glycoprotein synthesis. Composed of tunicamycins A, B, C and D, the compound competitively blocks the transfer of N-acetylglucosamine-1-phosphate (GlcNAc-1-P) from UDP-GlcNAc to dolichol-P (1,2). By preventing glycoprotein synthesis, tunicamycin inhibits the formation of the “viral coat” known as the tunica or capsid in both RNA and DNA viruses, and thus exhibits antiviral properties (3). Research studies have shown that tunicamycin will arrest cells in G1 phase, preventing them from entering S-phase and increasing the expression of PERK (4,5). Following N-linked glycosylation inhibition, tunicamycin induces autophagy in response to ER stress, ultimately upregulating CHOP and BIP (6,7).

  1. Takatsuki, A. et al. (1971) J Antibiot (Tokyo) 24, 215-23.
  2. Kaushal, G.P. and Elbein, A.D. (1986) Plant Physiol 82, 748-52.
  3. Hirano, T. et al. (1982) J Biochem 92, 765-73.
  4. Brewer, J.W. and Diehl, J.A. (2000) Proc Natl Acad Sci U S A 97, 12625-30.
  5. Harding, H.P. et al. (1999) Nature 397, 271-274.
  6. Ding, W.X. et al. (2007) J Biol Chem 282, 4702-10.
  7. Zinszner, H. et al. (1998) Genes Dev 12, 982-95.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
To Purchase # 12819S
Product # Size Price
12819S
5 mg $ 118