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9842
Tyrphostin AG 1478
Activators & Inhibitors
Chemical Modulators

Tyrphostin AG 1478 #9842

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Tyrphostin AG 1478: Image 1
Structure of Tyrphostin AG 1478
Tyrphostin AG 1478: Image 2
Western blot analysis of extracts from serum starved A431 cells, untreated or treated with indicated concentrations of Tyrphostin AG1478 for 30 minutes, followed by stimulation with EGF for 10 minutes. The EGFR activation was detected by using Phospho-EGF Receptor (Tyr1173) (53A5) Rabbit mAb #4407 (upper). EGF Receptor Antibody #2232 was used to detect the equal loading of EGFR (lower). Tyrphostin AG1478-mediated inhibition of EGF-induced phosphorylation of EGF Receptor at Tyr1173 is shown.

Product Usage Information

Tyrphostin AG 1478 is supplied as 400 μg powder. Store at or below -20ºC. Before use, dissolve powder in 1.23 ml DMSO to make a 1 mM

Tyrphostin AG 1478 stock solution. For working concentrations of 100 nM-200 nM, dilute DMSO stock 1:10,000 to 1:5,000. Treat cells with the desired concentration for 30 minutes.

Storage

Store lyophilized or in solution at -20ºC, desiccated. Protect from light. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight 315.76 g/mol
Purity >99%
Molecular Formula C16H14CIN3O2
CAS 175178-82-2
Solubility Soluble in DMSO at 10mg/ml.

Background

Tyrphostin AG 1478 is a tyrosine kinase inhibitor specifically selective to EGFR (ErbB1), with an IC50 of about 3 nM in vitro (1,2). Treatment of cell with 50-150 nM of AG 1478 can substantially block EGFR activiation in vivo (3). In addition to EGFR, AG 1478 also inhibits ErbB4 activation induced by radiation in cancer cells (4). Testing of AG 1478 alone or in combination with other treatments to assess anti-tumor and anti-fibrotic effectiveness has yielded promising results (5-8).
  1. Osherov, N. and Levitzki, A. (1994) Eur J Biochem 225, 1047-53.
  2. Levitzki, A. and Gazit, A. (1995) Science 267, 1782-8.
  3. Fan, Z. et al. (1995) J Cell Biol 131, 235-42.
  4. Bowers, G. et al. (2001) Oncogene 20, 1388-97.
  5. Nagane, M. et al. (2001) J Neurosurg 95, 472-9.
  6. Ishii, Y. et al. (2006) Am J Respir Crit Care Med 174, 550-6.
  7. Ellis, A.G. et al. (2006) Biochem Pharmacol 71, 1422-34.
  8. Rahman, M. et al. (2009) Breast Cancer Res Treat 113, 217-30.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
To Purchase # 9842
Cat. # Size Qty. Price
9842S
400 µg $ 33