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Activators & Inhibitors
Chemical Modulators

Vemurafenib #17531

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Vemurafenib: Image 1
Western blot analysis of extracts from SK-MEL-28 cells, untreated (-) or treated with Vemurafenib (24 hr) at the indicated concentrations, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370, p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695, Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb #9154, and MEK1/2 (D1A5) Rabbit mAb #8727.
Vemurafenib: Image 2
Chemical structure of Vemurafenib.

Product Usage Information

Vemurafenib is supplied as a lyophilized powder. For a 50 mM stock, reconstitute the 5 mg of powder in 204.12 μl of DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used between 0.5-50 μM for 24 hrs.


Store lyophilized or in solution at -20ºC, desiccated. Protect from light. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight 489.92 g/mol
Purity >99%
Molecular Formula C23H18CIF2N3O3S
CAS 918504-65-1
Solubility Soluble in DMSO at 100mg/ml.


Vemurafenib, also known as PLX4032, is an inhibitor of mutated BRAF (V600E) (1,2). The BRAF kinase is responsible for the activation of MEK and in turn, activating ERK and other downstream transcription factors involved in cell differentiation, proliferation, growth and apoptosis (1). The V600E mutation elevates the catalytic activity of BRAF which in turn renders it insensitive to negative feedback, thus causing hyperactivation of ERK signaling (2). Ultimately, the inhibition of mutated BRAF will cause a cascade of inhibitory actions against downstream targets, such as ERK and MEK1/2 (1,2).
  1. Cantwell-Dorris, E.R. et al. (2011) Mol Cancer Ther 10, 385-94.
  2. Joseph, E.W. et al. (2010) Proc Natl Acad Sci U S A 107, 14903-8.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
To Purchase # 17531
Cat. # Size Qty. Price
5 mg
$ 50