News from the Bench

Discover what’s going on at CST, receive our latest application notes and tips, read our science features, and learn about our products.

Subscribe

Santa Cruz discontinued a large number of its polyclonal products as a result of the USDA settlement that was made public May 19th 2016

Find CST Equivalent

To Purchase # 13043S

13043S 5 mg $99.00.0
$0.00

Questions?

Find answers on our FAQs page.

ANSWERS  

PhosphoSitePlus® Resource

  • Additional protein information
  • Analytical tools

LEARN MORE

Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Vincristine (10 nM, 18 hr; +), using Phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) (D13A11) XP® Rabbit mAb #2914 (upper) or Aurora A (1F8) Mouse mAb #12100 and COX IV (3E11) Rabbit mAb #4850 (lower).

Learn more about how we get our images

Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Vincristine (10 nM, 18 hr; +), using Phospho-NPM (Ser4) (D19C1) XP® Rabbit mAb #3520 (upper) or NPM Antibody #3542 (lower).

Learn more about how we get our images

Chemical structure of vincristine.

Learn more about how we get our images
Image

Product Usage Information

Vincristine is supplied as a lyophilized powder. For a 10 mM stock, reconstitute the 5 mg in 541.7 µl DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 10-1000 nM for 12-48 hr.


Storage: Store lyophilized or in solution at -20ºC, desiccated. Protect from light. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight:

923.04 g/mol


Purity:

>99%


Molecular Formula:

C46H56N4O10•H2SO4


Vincristine is a vinca alkaloid isolated from the plant Catharanthus roseus that acts as a mitotic inhibitor blocking microtubule assembly in vitro (1,2). Research studies demonstrated that vincristine binds to and inhibits the addition of tubulin dimers to the assembly end of steady-state microtubules in a dose-dependent manner (Ki = ~85 nM) (1). Additional research suggests that high concentrations of vincristine depolymerize microtubules (2). Disruption of mitotic spindle function has been shown to cause mitotic arrest, inhibit cell proliferation, and induce apoptosis in numerous cell types (1-6).


1.  Jordan, M.A. et al. (1985) Cancer Res 45, 2741-7.

2.  Jordan, M.A. et al. (1991) Cancer Res 51, 2212-22.

3.  Birk, M. et al. (2012) Int J Cancer 130, 798-807.

4.  Takano, Y. et al. (1993) Pathol Res Pract 189, 197-203.

5.  Tu, Y. et al. (2013) Int J Mol Med 31, 113-9.

6.  Groninger, E. et al. (2002) Int J Oncol 21, 1339-45.



For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.

13043
Vincristine