Chemical structure of vincristine.
Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Vincristine (10 nM, 18 hr; +), using Phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) (D13A11) XP® Rabbit mAb #2914 (upper) or Aurora A (1F8) Mouse mAb #12100 and COX IV (3E11) Rabbit mAb #4850 (lower).
Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Vincristine (10 nM, 18 hr; +), using Phospho-NPM (Ser4) (D19C1) XP® Rabbit mAb #3520 (upper) or NPM Antibody #3542 (lower).
Vincristine is supplied as a lyophilized powder. For a 10 mM stock, reconstitute the 5 mg in 541.7 µl DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 10-1000 nM for 12-48 hr.
Store lyophilized or in solution at -20ºC, desiccated. Protect from light. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.
|Molecular Weight||923.04 g/mol|
|Solubility||Soluble in DMSO at 60mg/ml and H2O at 25mg/ml.|
Vincristine is a vinca alkaloid isolated from the plant Catharanthus roseus that acts as a mitotic inhibitor blocking microtubule assembly in vitro (1,2). Research studies demonstrated that vincristine binds to and inhibits the addition of tubulin dimers to the assembly end of steady-state microtubules in a dose-dependent manner (Ki = ~85 nM) (1). Additional research suggests that high concentrations of vincristine depolymerize microtubules (2). Disruption of mitotic spindle function has been shown to cause mitotic arrest, inhibit cell proliferation, and induce apoptosis in numerous cell types (1-6).
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