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Santa Cruz discontinued a large number of its polyclonal products as a result of the USDA settlement that was made public May 19th 2016

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12520S 5 mg $53.00.0
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Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Vorinostat (SAHA) (18 hr; +) at the indicated concentrations, using Acetyl-Histone H3 (Lys14) Antibody #4318 (upper) or Histone H3 (D1H2) XP® Rabbit mAb #4499 (lower).

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Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Vorinostat (SAHA) (18 hr; +) at the indicated concentrations, using Acetyl-α-Tubulin (Lys40) (D20G3) XP® Rabbit mAb #5335 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower).

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Chemical structure of vorinostat (SAHA).

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Product Usage Information

Vorinostat is supplied as a lyophilized powder. For a 20 mM stock, reconstitute the 5 mg in 945.8 µl DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 1-10 µM for 2-24 hr. Soluble in DMSO at 66 mg/ml; soluble in ethanol at 2 mg/ml with slight warming; very poorly soluble in water with maximum solubility ~20-50 µM.


Storage: Store lyophilized or in solution at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight:

264.32 g/mol


Purity:

>99%


Molecular Formula:

C14H20N2O3


Vorinostat (SAHA) is a histone deacetylase (HDAC) inhibitor that acts by binding to and blocking the active site of the enzyme (1). Both class I and class II HDACs are inhibited by vorinostat at nanomolar concentrations (1-3). Research studies have shown that vorinostat causes the accumulation of acetylated histones, certain transcription factors, and other nonhistone proteins, which regulate gene expression and protein function. This leads to cell cycle arrest, differentiation, and/or apoptosis in many transformed cell types treated with micromolar concentrations of vorinostat (1,4-7).


1.  Marks, P.A. and Breslow, R. (2007) Nat Biotechnol 25, 84-90.

2.  Richon, V.M. et al. (1998) Proc Natl Acad Sci U S A 95, 3003-7.

3.  Moradei, O. et al. (2005) Curr Med Chem Anticancer Agents 5, 529-60.

4.  Kim, M.J. et al. (2012) Anticancer Res 32, 3161-8.

5.  Richon, V.M. et al. (2000) Proc Natl Acad Sci U S A 97, 10014-9.

6.  Butler, L.M. et al. (2000) Cancer Res 60, 5165-70.

7.  Lee, J.H. et al. (2010) Proc Natl Acad Sci U S A 107, 14639-44.



For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.

12520
Vorinostat (SAHA)