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12520
Vorinostat (SAHA)
Activators & Inhibitors
Chemical Modulators

Vorinostat (SAHA) #12520

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Vorinostat (SAHA): Image 1

Chemical structure of vorinostat (SAHA).

Vorinostat (SAHA): Image 2

Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Vorinostat (SAHA) (18 hr; +) at the indicated concentrations, using Acetyl-Histone H3 (Lys14) Antibody #4318 (upper) or Histone H3 (D1H2) XP® Rabbit mAb #4499 (lower).

Vorinostat (SAHA): Image 3

Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Vorinostat (SAHA) (18 hr; +) at the indicated concentrations, using Acetyl-α-Tubulin (Lys40) (D20G3) XP® Rabbit mAb #5335 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower).

Product Usage Information

Vorinostat is supplied as a lyophilized powder. For a 20 mM stock, reconstitute the 5 mg in 945.8 µl DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 1-10 µM for 2-24 hr. Soluble in DMSO at 66 mg/ml; soluble in ethanol at 2 mg/ml with slight warming; very poorly soluble in water with maximum solubility ~20-50 µM.

Storage

Store lyophilized or in solution at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight 264.32 g/mol
Purity

>99%

Molecular Formula

C14H20N2O3

CAS 149647-78-9
Solubility Soluble in DMSO at 66mg/ml and EtOH at 2mg/ml.

Background

Vorinostat (SAHA) is a histone deacetylase (HDAC) inhibitor that acts by binding to and blocking the active site of the enzyme (1). Both class I and class II HDACs are inhibited by vorinostat at nanomolar concentrations (1-3). Research studies have shown that vorinostat causes the accumulation of acetylated histones, certain transcription factors, and other nonhistone proteins, which regulate gene expression and protein function. This leads to cell cycle arrest, differentiation, and/or apoptosis in many transformed cell types treated with micromolar concentrations of vorinostat (1,4-7).

  1. Marks, P.A. and Breslow, R. (2007) Nat Biotechnol 25, 84-90.
  2. Richon, V.M. et al. (1998) Proc Natl Acad Sci U S A 95, 3003-7.
  3. Moradei, O. et al. (2005) Curr Med Chem Anticancer Agents 5, 529-60.
  4. Kim, M.J. et al. (2012) Anticancer Res 32, 3161-8.
  5. Richon, V.M. et al. (2000) Proc Natl Acad Sci U S A 97, 10014-9.
  6. Butler, L.M. et al. (2000) Cancer Res 60, 5165-70.
  7. Lee, J.H. et al. (2010) Proc Natl Acad Sci U S A 107, 14639-44.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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To Purchase # 12520S
Product # Size Price
12520S
5 mg $ 58