Western blot analysis of extracts from Jurkat cells, untreated (-) or treated with Etoposide #2200 (25 μM, 5 hr; +), either with or without Z-VAD(OMe)-FMK pretreatment (1 hr) at the indicated concentrations, using Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb #9496, Caspase-8 (1C12) Mouse mAb #9746, Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb #5625, and PARP (46D11) Rabbit mAb #9532.Learn more about how we get our images
Chemical structure of Z-VAD(OMe)-FMK.Learn more about how we get our images
Z-VAD(OMe)-FMK is supplied as a lyophilized powder. For a 10 mM stock, reconstitute the 1 mg of powder in 213.9 μl of DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used as a pre-treatment at 5-100 μM for 1 hr.
Store lyophilized or in solution at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.
The synthetic peptide Z-VAD(OMe)-FMK is a cell permeable, irreversible pan-caspase inhibitor that displays non-cytotoxic effects (1-4). Z-VAD(OMe)-FMK prevents caspase-3 from adopting its active form and thereby blocks apoptosis (5). Research studies show that Z-VAD(OMe)-FMK also inhibits other enzymes, including cathepsin B, PNGase, and picornaviral 2A proteinases (6-8). Additional studies demonstrate that Z-VAD(OMe)-FMK can inhibit mitogen-induced T cell proliferation (2,9,10).
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. XP is a registered trademark of Cell Signaling Technology, Inc.
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