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Western blot analysis of extracts from Jurkat cells, untreated (-) or treated with Etoposide #2200 (25 μM, 5 hr; +), either with or without Z-VAD(OMe)-FMK pretreatment (1 hr) at the indicated concentrations, using Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb #9496, Caspase-8 (1C12) Mouse mAb #9746, Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb #5625, and PARP (46D11) Rabbit mAb #9532.

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Chemical structure of Z-VAD(OMe)-FMK.

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Product Usage Information

Z-VAD(OMe)-FMK is supplied as a lyophilized powder. For a 10 mM stock, reconstitute the 1 mg of powder in 213.9 μl of DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used as a pre-treatment at 5-100 μM for 1 hr.

Storage: Store lyophilized or in solution at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight:

467.5 g/mol



Molecular Formula:


The synthetic peptide Z-VAD(OMe)-FMK is a cell permeable, irreversible pan-caspase inhibitor that displays non-cytotoxic effects (1-4). Z-VAD(OMe)-FMK prevents caspase-3 from adopting its active form and thereby blocks apoptosis (5). Research studies show that Z-VAD(OMe)-FMK also inhibits other enzymes, including cathepsin B, PNGase, and picornaviral 2A proteinases (6-8). Additional studies demonstrate that Z-VAD(OMe)-FMK can inhibit mitogen-induced T cell proliferation (2,9,10).

1.  Garcia-Calvo, M. et al. (1998) J Biol Chem 273, 32608-13.

2.  Lawrence, C.P. and Chow, S.C. (2012) Toxicol Appl Pharmacol 265, 103-12.

3.  Caserta, T.M. et al. (2003) Apoptosis 8, 345-52.

4.  Van Noorden, C.J. (2001) Acta Histochem 103, 241-51.

5.  Slee, E.A. et al. (1996) Biochem J 315 ( Pt 1), 21-4.

6.  Schotte, P. et al. (1999) FEBS Lett 442, 117-21.

7.  Misaghi, S. et al. (2006) Cell Death Differ 13, 163-5.

8.  Deszcz, L. et al. (2004) FEBS Lett 560, 51-5.

9.  Alam, A. et al. (1999) J Exp Med 190, 1879-90.

10.  Boissonnas, A. et al. (2002) Eur J Immunol 32, 3007-15.

Data Sheets & Documentation

For Research Use Only. Not For Use In Diagnostic Procedures.
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