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Activators & Inhibitors

Z-VAD(OMe)-FMK #60332

Western Blotting

Western blot analysis of extracts from Jurkat cells, untreated (-) or treated with Etoposide #2200 (25 μM, 5 hr; +), either with or without Z-VAD(OMe)-FMK pretreatment (1 hr) at the indicated concentrations, using Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb #9496, Caspase-8 (1C12) Mouse mAb #9746, Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb #5625, and PARP (46D11) Rabbit mAb #9532.

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Chemical structure of Z-VAD(OMe)-FMK.

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Z-VAD(OMe)-FMK is supplied as a lyophilized powder. For a 10 mM stock, reconstitute the 1 mg of powder in 213.9 μl of DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used as a pre-treatment at 5-100 μM for 1 hr.


Store lyophilized or in solution at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Molecular Weight:

467.5 g/mol



Molecular Formula:


The synthetic peptide Z-VAD(OMe)-FMK is a cell permeable, irreversible pan-caspase inhibitor that displays non-cytotoxic effects (1-4). Z-VAD(OMe)-FMK prevents caspase-3 from adopting its active form and thereby blocks apoptosis (5). Research studies show that Z-VAD(OMe)-FMK also inhibits other enzymes, including cathepsin B, PNGase, and picornaviral 2A proteinases (6-8). Additional studies demonstrate that Z-VAD(OMe)-FMK can inhibit mitogen-induced T cell proliferation (2,9,10).

  1. Garcia-Calvo, M. et al. (1998) J Biol Chem 273, 32608-13.
  2. Lawrence, C.P. and Chow, S.C. (2012) Toxicol Appl Pharmacol 265, 103-12.
  3. Caserta, T.M. et al. (2003) Apoptosis 8, 345-52.
  4. Van Noorden, C.J. (2001) Acta Histochem 103, 241-51.
  5. Slee, E.A. et al. (1996) Biochem J 315 ( Pt 1), 21-4.
  6. Schotte, P. et al. (1999) FEBS Lett 442, 117-21.
  7. Misaghi, S. et al. (2006) Cell Death Differ 13, 163-5.
  8. Deszcz, L. et al. (2004) FEBS Lett 560, 51-5.
  9. Alam, A. et al. (1999) J Exp Med 190, 1879-90.
  10. Boissonnas, A. et al. (2002) Eur J Immunol 32, 3007-15.
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.

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