Src Antibodies

Target Information

a proto-oncogenic cytoplasmic tyrosine kinase of the SRC family which is activated following engagement of many different classes of cellular receptors including immune response receptors, integrins and other adhesion receptors, receptor protein tyrosine kinases, G protein-coupled receptors as well as cytokine receptors. Highly expressed in certain fully differentiated cells such as neurons, platelets and macrophages. Participates in signaling pathways that control a diverse spectrum of biological activities including MAPK pathways regulating of growth and differentiation, gene transcription, RNA processing, immune responses, cell adhesion, cell cycle progression, apoptosis, migration, and transformation. Due to functional redundancy between members of the SRC kinase family, identification of the specific role of each SRC kinase is very difficult. SRC appears to be one of the primary kinases activated following engagement of plasma membrane receptors and plays a role in the activation and regulation of other protein tyrosine kinases including PTK2, EGFR, FLT4, and DDR2. Receptor clustering or dimerization leads to recruitment of SRC to the receptor complexes where it phosphorylates the tyrosine residues within the receptor cytoplasmic domains. Recruited to activated integrins by PTK2B/PYK2, thereby phosphorylating CBL, which in turn induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function. Plays an important role in the regulation of cytoskeletal organization through phosphorylation proteins including AFAP1, RHOA, cortactin (CTTN) and villin. Phosphorylation of AFAP1 allows the SRC SH2 domain to bind AFAP1 and to localize to actin filaments. Cytoskeletal reorganization is also controlled through the phosphorylation of CTTN. When cells adhere via focal adhesions to the extracellular matrix, signals are transmitted by integrins into the cell resulting in tyrosine phosphorylation of a number of focal adhesion proteins, including PTK2/FAK1. In addition to phosphorylating focal adhesion proteins, SRC is also active at the sites of cell-cell contact adherens junctions and phosphorylates substrates such as beta-catenin (CTNNB1). SRC phosphorylates RNA-binding proteins including KHDRBS1, a major tyrosine phosphoprotein in transformed and growth factor treated cells. Its binding to RNA is significantly impaired following SRC-mediated phosphorylation, suggesting that it may be involved in regulating pre-mRNA-processing. Phosphorylates STAT1 and STAT3, leading to increased DNA binding activity of these transcription factors. Plays a role in EGF-mediated calcium-activated chloride channel activation. Involved in beta-arrestin (ARRB1 and ARRB2) desensitization through phosphorylation and activation of GRK2, leading to beta-arrestin phosphorylation and internalization. Plays a critical role in the stimulation of the CDK20/ERK1 mitogen-activated protein kinase cascade by epidermal growth factor. Plays an important role in osteoclastic bone resorption in conjunction with PYK2. Both the formation of a SRC-PYK2 complex and SRC kinase activity are necessary for this function. Promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase. Required for podosome formation. Phosphorylation of an activation loop tyrosine activates the enzyme; phosphorylation of a tyrosine in the C-terminus by Csk inhibits the enzyme. Expressed ubiquitously. Platelets, neurons and osteoclasts express 5-fold to 200-fold higher levels than most other tissues. 2 alternatively spliced human isoforms have been reported. Note: This description may include information from UniProtKB.

Alternate Names

ASV; c-SRC; p60-Src; pp60c-src; Proto-oncogene c-Src; Proto-oncogene tyrosine-protein kinase Src; protooncogene SRC, Rous sarcoma; SRC; SRC proto-oncogene, non-receptor tyrosine kinase; SRC1; THC6; tyrosine kinase pp60c-src; tyrosine-protein kinase SRC-1; v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog; v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)