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Product Usage Information

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibodies. Protect from light. Do not freeze.

Specificity / Sensitivity

PCNA (PC10) Mouse mAb (PE Conjugate) detects endogenous levels of total PCNA protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant Protein A-PCNA fusion protein obtained from pC2T.

Product Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated PCNA (PC10) Mouse mAb #2586.

Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication (1). Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix (2). Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA (reviewed in 3). PCNA protein expression is a well-accepted marker of proliferation.

1.  Kelman, Z. and O'Donnell, M. (1995) Nucleic Acids Res 23, 3613-20.

2.  Krishna, T.S. et al. (1994) Cell 79, 1233-43.

3.  Maga, G. and Hubscher, U. (2003) J Cell Sci 116, 3051-60.

Entrez-Gene Id 5111
Swiss-Prot Acc. P12004

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
DRAQ5 is a registered trademark of Biostatus Limited.

PCNA (PC10) Mouse mAb (PE Conjugate)