The Apoptosis and Proliferation Alexa Fluor® 488 Conjugated Antibody Sampler Kit provides an economical means to study apoptosis and proliferation status using known markers without the need for a fluorescent secondary antibody.
Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of caspase-3 protein only when cleaved at Asp175. Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of the large fragment (89 kDa) of human PARP1 protein produced by caspase cleavage. This antibody does not recognize full length PARP1 or other PARP isoforms. Ki-67 (D3B5) Rabbit mAb (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of total Ki-67 protein.
Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp175 of human caspase-3 protein, a synthetic peptide corresponding to residues surrounding Asp214 of human PARP protein, or a recombinant protein specific to the amino terminus of Ki-67 protein.
Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). PARP is involved in DNA repair in response to environmental stress (2). Ki-67 is a nuclear nonhistone protein that is universally expressed among proliferating cells and absent in quiescent cells (3,4). Ki-67 detects proliferating cells in G1, S, G2, and mitosis, but not in the G0 resting phase.
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