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12145
Apoptosis and Proliferation Alexa Fluor® 488 Conjugated Antibody Sampler Kit
Antibody Conjugates
Antibody Sampler Kit

Apoptosis and Proliferation Alexa Fluor® 488 Conjugated Antibody Sampler Kit #12145

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Apoptosis and Proliferation Alexa Fluor® 488 Conjugated Antibody Sampler Kit: Image 1
Confocal immunofluorescent analysis of normal rat colon using Ki-67 (D3B5) Rabbit mAb (Alexa Fluor® 488 Conjugate) (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Apoptosis and Proliferation Alexa Fluor® 488 Conjugated Antibody Sampler Kit: Image 2
Confocal immunofluorescent analysis of HeLa cells using Ki-67 (D3B5) Rabbit mAb (Alexa Fluor® 488 Conjugate) (green). Actin filaments were labeled with DY-554 phalloidin (red).
Apoptosis and Proliferation Alexa Fluor® 488 Conjugated Antibody Sampler Kit: Image 3
Flow cytometric analysis of Jurkat cells using Ki-67 (D3B5) Rabbit mAb (Alexa Fluor® 488 Conjugate) and Propidium Iodide (PI) /RNase Staining Solution #4087 (DNA content).
Apoptosis and Proliferation Alexa Fluor® 488 Conjugated Antibody Sampler Kit: Image 4
Confocal immunofluorescent analysis of Cos cells using GAPDH (14C10) Rabbit mAb (Alexa Fluor® 488 Conjugate) #3906 (green, left) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 488 Conjugate (green, right). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Apoptosis and Proliferation Alexa Fluor® 488 Conjugated Antibody Sampler Kit: Image 5
Flow cytometric analysis of Jurkat cells, using Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 488 Conjugate) (red) compared to Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #4803, untreated (blue) or IFN-α-treated (green).
Apoptosis and Proliferation Alexa Fluor® 488 Conjugated Antibody Sampler Kit: Image 6
Flow cytometric analysis of Jurkat cells, using Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 488 Conjugate) (red) compared to Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #4071, untreated (green) or LY294002- and wortmannin-treated (blue).
Apoptosis and Proliferation Alexa Fluor® 488 Conjugated Antibody Sampler Kit: Image 7
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with staurosporine #9953 (1 μM for 3 hours, right), using Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) (green). Actin filaments were labeled with DY-554 phalloidin (red).
Apoptosis and Proliferation Alexa Fluor® 488 Conjugated Antibody Sampler Kit: Image 8

Flow cytometric analysis of Jurkat cells, untreated (blue) or treated with Etoposide (25uM overnight; green) using Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) (solid lines) or concentration-matched

Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 488 Conjugate) #2975 (dashed lines).


Apoptosis and Proliferation Alexa Fluor® 488 Conjugated Antibody Sampler Kit: Image 9
Confocal immunofluorescent analysis of HeLa cells, untreated (left), or treated with staurosporine #9953 (right), using Cleaved Caspase-3 (Asp175) (D3E9) (Alexa Fluor® 488 Conjugate) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Apoptosis and Proliferation Alexa Fluor® 488 Conjugated Antibody Sampler Kit: Image 10
Flow cytometric analysis of Jurkat cells, untreated (blue) or treated with Etoposide (25 uM, overnight; green) using Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb (Alexa Fluor® 488 Conjugate) (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 488 Conjugate) #2975 (dashed lines).
Inquiry Info.# 12145

Kit Usage Information

Protocols

Product Description

The Apoptosis and Proliferation Alexa Fluor® 488 Conjugated Antibody Sampler Kit provides an economical means to study apoptosis and proliferation status using known markers without the need for a fluorescent secondary antibody.

Specificity / Sensitivity

Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of caspase-3 protein only when cleaved at Asp175. Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of the large fragment (89 kDa) of human PARP1 protein produced by caspase cleavage. This antibody does not recognize full length PARP1 or other PARP isoforms. Ki-67 (D3B5) Rabbit mAb (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of total Ki-67 protein.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp175 of human caspase-3 protein, a synthetic peptide corresponding to residues surrounding Asp214 of human PARP protein, or a recombinant protein specific to the amino terminus of Ki-67 protein.

Background

Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). PARP is involved in DNA repair in response to environmental stress (2). Ki-67 is a nuclear nonhistone protein that is universally expressed among proliferating cells and absent in quiescent cells (3,4). Ki-67 detects proliferating cells in G1, S, G2, and mitosis, but not in the G0 resting phase.
  1. Fernandes-Alnemri, T. et al. (1994) J Biol Chem 269, 30761-4.
  2. Satoh, M.S. and Lindahl, T. (1992) Nature 356, 356-8.
  3. Gerdes, J. et al. (1983) Int J Cancer 31, 13-20.
  4. Weigel, M.T. and Dowsett, M. (2010) Endocr Relat Cancer 17, R245-62.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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