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9667
Apoptosis Marker: Cleaved Caspase-3 (Asp175) Antibody (Fluorescein Conjugate)
Antibody Conjugates

Apoptosis Marker: Cleaved Caspase-3 (Asp175) Antibody (Fluorescein Conjugate) #9667

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# Product Name Application Reactivity
  • IF
  • F
H M R Mk

Supporting Data

REACTIVITY
SENSITIVITY Endogenous
MW (kDa)
SOURCE Rabbit

Application Key:

  • W-Western
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Specificity / Sensitivity

Cleaved Caspase-3 (Asp175) Antibody (Fluorescein Conjugate) detects endogenous levels of cleaved caspase-3 and serves as a useful marker for detecting apoptotic cells by immunofluorescence. For fluorescent detection, use excitation 494 nm and emission 520 nm.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues around the cleavage site of caspase-3 at Asp175. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires the aspartic acid residue at the P1 position (2).

  1. Fernandes-Alnemri, T. et al. (1994) J Biol Chem 269, 30761-4.
  2. Nicholson, D.W. et al. (1995) Nature 376, 37-43.

Pathways & Proteins

Explore pathways + proteins related to this product.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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