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Apoptosis Marker: Cleaved Caspase-3 (Asp175) Antibody (Fluorescein Conjugate)

Apoptosis Marker: Cleaved Caspase-3 (Asp175) Antibody (Fluorescein Conjugate) #9667

Inquiry Info. # 9667

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Product Specifications

REACTIVITY
SENSITIVITY	Endogenous
MW (kDa)
SOURCE	Rabbit

Product Information

Specificity / Sensitivity

Apoptosis Marker: Cleaved Caspase-3 (Asp175) Antibody (Fluorescein Conjugate) detects endogenous levels of cleaved caspase-3 and serves as a useful marker for detecting apoptotic cells by immunofluorescence. For fluorescent detection, use excitation 494 nm and emission 520 nm.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues around the cleavage site of caspase-3 at Asp175. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Caspase-3 (CPP-32, Apopain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires the aspartic acid residue at the P1 position (2).

Alternate Names

Apopain; CASP-3; CASP3; caspase 3; caspase 3, apoptosis-related cysteine peptidase; caspase 3, apoptosis-related cysteine protease; Caspase-3; Caspase-3 subunit p12; Caspase-3 subunit p17; caspase3; CPP-32; CPP32; CPP32B; Cysteine protease CPP32; PARP cleavage protease; procaspase3; Protein Yama; SCA-1; SREBP cleavage activity 1; Yama

Database Links

UniProt ID: P42574

Entrez-Gene Id: 836

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For Research Use Only. Not for Use in Diagnostic Procedures.

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