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Render Timestamp: 2024-11-04T09:48:57.151Z
Commit: 23cb9f61fe67e1e9093fd644a533c4ff516a6463
XML generation date: 2024-09-20 06:22:23.519
Product last modified at: 2024-09-20T07:02:18.862Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

CD11c (N418) Hamster mAb (APC-Cy7® Conjugate) #37664

Filter:
  • F

    Supporting Data

    REACTIVITY M
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Hamster (armenian) IgG
    Application Key:
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Description

    This Cell Signaling Technology antibody is conjugated to APC-Cy7® and tested in-house for direct flow cytometric analysis in mouse cells.

    Product Usage Information

    For optimal flow cytometry results, we recommend 0.25 μg of antibody per test.

    Application Dilution
    Flow Cytometry (Live) 1:80

    Storage

    Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 6 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze.

    Protocol

    Specificity / Sensitivity

    CD11c (N418) Hamster mAb (APC-Cy7® Conjugate) recognizes endogenous levels of total CD11c protein. This antibody detects an epitope within the extracellular domain.

    Species Reactivity:

    Mouse

    Source / Purification

    This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

    Background

    CD11c (integrin αX, ITGAX) is a transmembrane glycoprotein that forms an α/β heterodimer with CD18 (integrin β2), which interacts with a variety of extracellular matrix molecules and cell surface proteins (1). CD11c is primarily used as a dendritic cell marker. Dendritic cells can be classified into two major types: CD11c+ conventional dendritic cells that specialize in antigen presentation, and CD11c- plasmacytoid dendritic cells that specialize in type I interferon production (2, 3). CD11c expression has also been observed on activated NK cells, subsets of B cells, monocytes, granulocytes, and some B cell malignancies including hairy cell leukemia (4-7).
    The N418 antibody is widely used as a marker for CD11c expression on the cell types mentioned above (8,9).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    Cy and CyDye are registered trademarks of GE Healthcare.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.