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CD62L/L-Selectin (MEL-14) Rat mAb (APC Conjugate)
Antibody Conjugates
Monoclonal Antibody

CD62L/L-Selectin (MEL-14) Rat mAb (APC Conjugate) #46592

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Flow Cytometry Image 1 - CD62L/L-Selectin (MEL-14) Rat mAb (APC Conjugate)

Flow cytometric analysis of live mouse splenocytes using CD62L/L-Selectin (MEL-14) Rat mAb (APC Conjugate) and co-stained with CD44 (IM7) Rat mAb (FITC Conjugate) #53289 (right), compared to concentration-matched Rat Isotype Control (APC Conjugate) (left).

To Purchase # 46592S
Product # Size Price
100 µg $ 199

Supporting Data

MW (kDa)
Source/Isotype Rat IgG2a kappa

Application Key:

  • W-Western
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Description

This Cell Signaling Technology antibody is conjugated to APC and tested in-house for direct flow cytometric analysis in mouse cells.

Product Usage Information

For optimal flow cytometry results, we recommend 0.06μg of antibody per test.

Application Dilution
Flow Cytometry 1:300


Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. Do not aliquot the antibody. Protect from light. Do not freeze.



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Flow Cytometry, Live Cell Protocol for Directly Conjugated Antibodies

A. Solutions and Reagents

NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.

  1. 1X Phosphate Buffered Saline (PBS): To prepare 1 L 1X PBS: add 100 ml 10X PBS (#12528) to 900 ml water, mix.
  2. Antibody Dilution Buffer: Purchase ready-to-use Flow Cytometry Antibody Dilution Buffer (#13616), or prepare a 0.5% BSA PBS buffer by dissolving 0.5 g Bovine Serum Albumin (BSA) (#9998) in 100 ml 1X PBS. Store at 4°C.

NOTE: When including fluorescent cellular dyes in your experiment (including viability dyes, DNA dyes, etc.), please refer to the dye product page for the recommended protocol. Visit for a full listing of cellular dyes validated for use in flow cytometry.

B. Immunostaining

NOTE: Count cells using a hemocytometer or alternative method.

NOTE: If using whole blood, lyse red blood cells and wash by centrifugation prior to Immunostaining.

NOTE: Optimal centrifugation conditions will vary depending upon cell type and reagent volume. Generally, 150-300g for 1-5 minutes will be sufficient to pellet the cells.

  1. Aliquot desired number of cells into tubes or wells. (Generally, 5x105 to 1x106 cells per assay.)
  2. Pellet cells by centrifugation and remove supernatant.
  3. Resuspend cells in 100 µl of diluted primary antibody, prepared in Antibody Dilution Buffer at a recommended dilution or as determined via titration.
  4. Incubate for 30 min to 1 hr on ice. Protect from light.
  5. Wash by centrifugation in Antibody Dilution Buffer. Discard supernatant. Repeat.
  6. Resuspend cells in 200-500 µl of Antibody Dilution Buffer and analyze on flow cytometer.

posted June 2017

revised June 2020

Protocol Id: 1504

Specificity / Sensitivity

CD62L/L-Selectin (MEL-14) Rat mAb (APC Conjugate) recognizes endogenous levels of total mouse CD62L protein.

Species Reactivity:


Source / Purification

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.


L-selectin (CD62L, MEL-14, LAM1, SELL) is a cell adhesion molecule, responsible for homing and mediating the binding of lymphocytes to high endothelial venules (HEV) in secondary lymphoid tissues (1-5). It is a commonly used marker for distinguishing naive and memory T cells from effector T cells (6).

  1. Gallatin, W.M. et al. Nature 304, 30-4.
  2. Tedder, T.F. et al. (1989) J Exp Med 170, 123-33.
  3. Tedder, T.F. et al. (1990) J Immunol 144, 532-40.
  4. Berg, E.L. et al. (1991) J Cell Biol 114, 343-9.
  5. Finger, E.B. et al. (1996) Nature 379, 266-9.
  6. Sallusto, F. et al. (1999) Nature 401, 708-12.

Pathways & Proteins

Explore pathways + proteins related to this product.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.