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46592
CD62L/L-Selectin (MEL-14) Rat mAb (APC Conjugate)
Antibody Conjugates

CD62L/L-Selectin (MEL-14) Rat mAb (APC Conjugate) #46592

APPLICATIONS

REACTIVITY SENSITIVITY MW (kDa) Isotype
M Endogenous Rat IgG2a, kappa
Flow Cytometry

Flow cytometric analysis of live mouse splenocytes using CD62L/L-Selectin (MEL-14) Rat mAb (APC Conjugate) and co-stained with CD44 (IM7) Rat mAb (FITC Conjugate) #53289 (right), compared to concentration-matched Rat Isotype Control (APC Conjugate) (left).

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Flow Cytometry

A. Solutions and Reagents

NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.

  1. 20X Phosphate Buffered Saline (PBS): (#9808) To prepare 1 L 1X PBS: add 50 ml 20X PBS to 950 ml dH2O, mix.
  2. Incubation Buffer: Dissolve 0.5 g Bovine Serum Albumin (BSA) (#9998) in 100 ml 1X PBS. Store at 4°C.

B. Immunostaining

  1. Count cells using a hemacytometer or alternative method.
  2. Aliquot 0.5-1x106 cells into each assay tube (by volume).
  3. Add 2-3 ml Incubation Buffer to each tube and rinse by centrifugation. Repeat.
  4. Resuspend cells in 100 µl of diluted primary antibody (prepared in incubation buffer at the recommended dilution).
  5. Incubate for 1 hr at room temperature.
  6. Wash by centrifugation in 2-3 ml incubation buffer.
  7. Resuspend cells in the appropriate volume of PBS and analyze on flow cytometer.

posted June 2017

Protocol Id: 1504

For optimal flow cytometry results, we recommend 0.06μg of antibody per test.

Application Dilutions
Flow Cytometry 1:300
Storage:

Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 6 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze.

CD62L/L-Selectin (MEL-14) Rat mAb (APC Conjugate) recognizes endogenous levels of total mouse CD62L protein.

Species Reactivity:

Mouse

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

This Cell Signaling Technology antibody is conjugated to APC and tested in-house for direct flow cytometric analysis in mouse cells.

L-selectin (CD62L, MEL-14, LAM1, SELL) is a cell adhesion molecule, responsible for homing and mediating the binding of lymphocytes to high endothelial venules (HEV) in secondary lymphoid tissues (1-5). It is a commonly used marker for distinguishing naive and memory T cells from effector T cells (6).

  1. Gallatin, W.M. et al. Nature 304, 30-4.
  2. Tedder, T.F. et al. (1989) J Exp Med 170, 123-33.
  3. Tedder, T.F. et al. (1990) J Immunol 144, 532-40.
  4. Berg, E.L. et al. (1991) J Cell Biol 114, 343-9.
  5. Finger, E.B. et al. (1996) Nature 379, 266-9.
  6. Sallusto, F. et al. (1999) Nature 401, 708-12.
Entrez-Gene Id
20343
Swiss-Prot Acc.
P18337
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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To Purchase # 46592S

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Product # Size Price
46592S
100 µg $ 199.0