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  3. Cleaved PARP (Asp214) Antibody (Human Specific) (Fluorescein Conjugate)

Cleaved PARP (Asp214) Antibody (Human Specific) (Fluorescein Conjugate) #9547

Inquiry Info. # 9547

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    Product Specifications

    REACTIVITY
    SENSITIVITY Endogenous
    MW (kDa)
    SOURCE Rabbit

    Product Information

    Specificity / Sensitivity

    Cleaved PARP (Asp214) Antibody (Human Specific) (Fluorescein Conjugate) detects endogenous levels of the large fragment of PARP1 (89 kDa) resulting from cleavage at aspartic acid 214. This antibody conjugate is a useful marker for detecting apoptotic cells by immunofluorescence. For fluorescence detection use excitation 494, emission 520 (FITC filter set compatible).

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to carboxy-terminal residues surrounding Asp214 in human PARP. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA-binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).

    (This product is sold under license from Promega Corp., U.S. Patent No. 6,350,452.)

    Alternate Names

    ADP-ribosyltransferase (NAD+); ADP-ribosyltransferase diphtheria toxin-like 1; ADP-ribosyltransferase NAD(+); ADPRT; ADPRT 1; ADPRT1; ARTD1; DNA ADP-ribosyltransferase PARP1; NAD(+) ADP-ribosyltransferase 1; pADPRT-1; PARP; PARP-1; PARP1; poly (ADP-ribose) polymerase; poly (ADP-ribose) polymerase 1; poly (ADP-ribose) polymerase family, member 1; Poly [ADP-ribose] polymerase 1; poly(ADP-ribose) polymerase; poly(ADP-ribose) polymerase 1; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase; Poly[ADP-ribose] synthase 1; PPOL; Protein poly-ADP-ribosyltransferase PARP1

    Database Links

    UniProt ID: P09874


    Entrez-Gene Id: 142


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