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12427
Epitope Tag Alexa Fluor® 488 Conjugated Antibody Sampler Kit
Antibody Conjugates
Antibody Sampler Kit

Epitope Tag Alexa Fluor® 488 Conjugated Antibody Sampler Kit #12427

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Product Description

The Epitope Tag Alexa Fluor® 488 Conjugated Antibody Sampler Kit provides an economical means to monitor the presence of proteins labeled with HA, GST, Myc, or DYKDDDDK (FLAG) tags without the need for a fluorescent secondary antibody.

Specificity / Sensitivity

GST (26H1) Mouse mAb (HRP Conjugate) recognizes transfected glutathione S-transferase (GST) fusion proteins. HA-Tag (6E2) Mouse mAb (HRP Conjugate) recognizes proteins containing the HA epitope tag. The antibody recognizes the HA-tag fused to either the amino or carboxy terminus of targeted proteins in transfected mammalian cells. Myc-Tag (9B11) Mouse mAb (HRP Conjugate) recognizes transfected proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag fused to either the amino or carboxy terminus of targeted proteins in transfected mammalian cells. His-Tag (D3I1O) XP® Rabbit mAb (HRP Conjugate) recognizes recombinant proteins containing the 6xHis epitope tag. The antibody recognizes the 6xHis-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. S-Tag (D2K2V) XP® Rabbit mAb (HRP Conjugate) recognizes transfected levels of total S-Tag protein

Source / Purification

Monoclonal antibodies are produced by immunizing animals with a synthetic peptide containing the influenza hemagglutinin epitope (YPYDVPDYA), a synthetic peptide corresponding to residues 410-419 of human c-Myc (EQKLISEEDL), or a GST fusion protein.

Polyclonal antibodies are produced by immunizing animals with a synthetic DYKDDDDK peptide. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Background

Epitope tags are useful for the labeling and detection of proteins using flow cytometry and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. HA tag is derived from an epitope of the influenza hemagglutinin protein, which has been extensively used as a general epitope tag in expression vectors (1). Glutathione S-transferase (GST) is a widely used fusion partner, since it provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Numerous vectors containing GST-Tag have been developed for both prokaryotic and eukaryotic systems over the past decade (2-4). Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect, and mammalian cell systems (5). DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion (6).

  1. Field, J. et al. (1988) Mol Cell Biol 8, 2159-65.
  2. Guan, K.L. and Dixon, J.E. (1991) Anal Biochem 192, 262-7.
  3. Davies, A.H. et al. (1993) Biotechnology (N Y) 11, 933-6.
  4. Yu, J. et al. (1998) Mol Cell Biol 18, 1379-87.
  5. Munro, S. and Pelham, H.R. (1984) EMBO J 3, 3087-93.
  6. Brizzard, B.L. et al. (1994) Biotechniques 16, 730-5.

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.
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Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.
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