Render Target: STATIC
Render Timestamp: 2024-12-12T11:42:54.734Z
Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-11-04 23:07:08.150
Product last modified at: 2024-12-06T23:30:10.276Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

G4S Linker (E7O2V) Rabbit mAb (PE Conjugate) #38907

Filter:
  • F

    Supporting Data

    REACTIVITY H All
    SENSITIVITY Transfected Only
    MW (kDa)
    Source/Isotype Rabbit IgG
    Application Key:
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 
    • All-All Species Expected 

    Product Information

    Product Description

    This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) fluorescent dye under optimal conditions and tested in-house for direct flow cytometric analysis in human cells. This antibody conjugate is expected to exhibit the same reactivity as the unconjugated G4S Linker (E7O2V) Rabbit mAb #71645, which is expected to react with cell surface-expressed CARs of varying specificity containing a G4S linker within the scFv of the extracellular domain.

    Product Usage Information

    Application Dilution
    Flow Cytometry (Live) 1:50

    Storage

    Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

    Protocol

    Specificity / Sensitivity

    G4S Linker (E7O2V) Rabbit mAb (PE Conjugate) recognizes exogenously expressed levels of scFv-based CARs containing a G4S linker.

    Species Reactivity:

    Human, All Species Expected

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide containing three Gly4Ser repeats.

    Background

    The poly-Glycine-Serine (G4S) linker is a type of flexible, unstructured synthetic peptide linker sequence often leveraged to connect the variable heavy (VH) domain and variable light (VL) domain of single-chain variable fragments (scFvs) and chimeric antigen receptors (CARs) that utilize an extracellular domain scFv for target antigen recognition. The linker itself consists of a core pentapeptide sequence, Gly-Gly-Gly-Gly-Ser, that is repeated and commonly found as either a 15-mer (G4S)3 or 20-mer (G4S)4 within scFv-based CARs and scFv fragments. The linker sequence length plays a role in controlling scFv stability and the noncovalent association between the VH and VL domains (1,2).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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