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GAPDH (14C10) Rabbit Monoclonal Antibody (Biotinylated) #5014

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  • WB

    Product Specifications

    REACTIVITY H M R Mk B Pg
    SENSITIVITY Endogenous
    MW (kDa) 37
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 
    • B-Bovine 
    • Pg-Pig 

    Product Information

    Product Description

    This Cell Signaling Technology® antibody is the biotinylated version of the unconjugated GAPDH (14C10) Rabbit Monoclonal Antibody #2118 and is expected to exhibit the same species cross-reactivity. The concentration of the biotinylated antibody is 500 ug/ml. Peptide ELISA data were generated using the biotinylated antibody. Western blot data were generated using the unconjugated antibody.
    MW (kDa) 37

    Product Usage Information

    Biotinylated antibodies are ideal for immunoassay technologies and high-throughput ELISA platforms that require antibody pairs where both antibodies are from the same host. Platforms utilizing biotinylated antibodies include, but are not limited to, MSD, xMAP, Quanterix Simoa, AlphaLISA, AlphaScreen, HTRF, LANCE, and TR-FRET.

    Optimal dilutions/working concentrations should be determined by the end user. Please contact us if you require the antibody clone biotinylated at a different concentration, a carrier-free formulation, or a more customized packaging solution.

    Storage

    Supplied in 140 mM NaCl, 3 mM KCI, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    GAPDH (14C10) Rabbit Monoclonal Antibody (Biotinylated) detects endogenous levels of total GAPDH protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey, Bovine, Pig

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide near the carboxy terminus of human GAPDH.

    Background

    Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Though differentially expressed from tissue to tissue (1), GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH mRNA and protein levels are often measured as controls in experiments quantifying specific changes in expression of other targets. Recent work has elucidated roles for GAPDH in apoptosis (2), gene expression (3), and nuclear transport (4). GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer's diseases (4,5).

    Alternate Names

    aging-associated gene 9 protein; epididymis secretory sperm binding protein Li 162eP; G3P; G3PD; GAPD; GAPDH; glyceraldehyde 3-phosphate dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase; HEL-S-162eP; MGC88685; OCAS, p38 component; Oct1 coactivator in S phase, 38 Kd component; Peptidyl-cysteine S-nitrosylase GAPDH

    For Research Use Only. Not for Use in Diagnostic Procedures.
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