GPNMB (E4D7P) Rabbit Monoclonal Antibody (Alexa Fluor® 647 Conjugate) #60293
- IHC
Product Specifications
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Description
Fluorescent Properties
- ← Excitation: 650 nm ← Emission: 668 nm
Product Usage Information
| Application | Dilution |
|---|---|
| Immunohistochemistry (Paraffin) | 1:100 - 1:400 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
While typical GPNMB expression is seen in tissues including skin, heart, kidney, lung, liver, and skeletal muscle (3,6), research studies show elevated GPNMB expression often contributes to the metastatic phenotype in numerous cancers (reviewed in 7). GPNMB is typically localized to intracellular compartments in normal cells (1,8), but investigators found it primarily on the cell surface of tumor cells (9,10). Differential localization and expression, and the role of GPNMB as a tumor promoter in many cancer types make this protein a viable therapeutic target (11).
The GPNMB ectodomain can be cleaved by matrix metalloproteinases and shed from the cell surface (12). Research studies identify the sheddase ADAM10 as one peptidase responsible for cleavage of the GPNMB ectodomain at the surface of breast cancer cells. Shedded GPNMB ectodomains may promote angiogenesis by inducing endothelial cell migration (13).
- Tomihari, M. et al. (2009) Exp Dermatol 18, 586-95.
- Sheng, M.H. et al. (2012) PLoS One 7, e35280.
- Shikano, S. et al. (2001) J Biol Chem 276, 8125-34.
- Li, B. et al. (2010) FASEB J 24, 4767-81.
- Patel-Chamberlin, M. et al. (2011) Kidney Int 79, 1138-48.
- Bandari, P.S. et al. (2003) Regul Pept 111, 169-78.
- Maric, G. et al. (2013) Onco Targets Ther 6, 839-52.
- Ripoll, V.M. et al. (2007) J Immunol 178, 6557-66.
- Tse, K.F. et al. (2006) Clin Cancer Res 12, 1373-82.
- Rose, A.A. et al. (2010) Clin Cancer Res 16, 2147-56.
- Keir, C.H. and Vahdat, L.T. (2012) Expert Opin Biol Ther 12, 259-63.
- Furochi, H. et al. (2007) FEBS Lett 581, 5743-50.
- Rose, A.A. et al. (2010) PLoS One 5, e12093.
Alternate Names
glycoprotein (transmembrane) nmb; glycoprotein NMB; glycoprotein nmb-like protein; glycoprotein nonmetastatic melanoma protein B; GPNMB; Hematopoietic growth factor inducible neurokinin-1 type; HGFIN; NMB; osteoactivin; PLCA3; Transmembrane glycoprotein HGFIN; Transmembrane glycoprotein NMB
Limited Uses
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