Cell Signaling Technology Logo - Extra Large
Recombinant Flag
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

HPK1 (E1C3L) Rabbit Monoclonal Antibody (PE Conjugate) #40187

Filter:
  • F

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Rabbit IgG
    Application Key:
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Description

    This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometric analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated HPK1 (E1C3L) Rabbit mAb #46510.

    Product Usage Information

    Application Dilution
    Flow Cytometry (Fixed/Permeabilized) 1:50

    Storage

    Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

    Protocol

    Specificity / Sensitivity

    HPK1 (E1C3L) Rabbit Monoclonal Antibody (PE Conjugate) recognizes endogenous levels of total HPK1 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human HPK1 protein.

    Background

    Hematopoietic progenitor kinase 1 (HPK1) is a member of the germinal center kinases that is predominantly expressed in hematopoietic cells. HPK1 is a key component linking T or B cell receptors to SAPK/JNK and IkappaB kinase pathways in lymphocytes (1-3). Through its proline-rich motif, HPK1 associates with multiple SH3 domain-containing adaptor proteins, including GRB2, Nck, Crk, SLP-76, and BLNK. Phosphorylation of Tyr379 by Syk is necessary for HPK1 association with BLNK and SLP-76, as well as for HPK1 activity (4,5). Caspase cleavage of HPK1 also modulates the biological function of HPK1 (3,6,7).

    Alternate Names

    Hematopoietic progenitor kinase; hematopoietic progenitor kinase 1; HPK1; M4K1; MAP4K1; MAPK/ERK kinase kinase kinase 1; MEK kinase kinase 1; MEKKK 1; Mitogen-activated protein kinase kinase kinase kinase 1

    For Research Use Only. Not for Use in Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.