REACTIVITY | M |
SENSITIVITY | Endogenous |
MW (kDa) | |
Source/Isotype | Hamster (Syrian) IgG |
Product Information
For optimal flow cytometry results, we recommend 0.06 μg of antibody per test.
Application | Dilution |
---|---|
Flow Cytometry (Live) | 1:300 |
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
NOTE: When including fluorescent cellular dyes in your experiment (including viability dyes, DNA dyes, etc.), please refer to the dye product page for the recommended protocol. Visit www.cellsignal.com for a full listing of cellular dyes validated for use in flow cytometry.
NOTE: Count cells using a hemocytometer or alternative method.
NOTE: If using whole blood, lyse red blood cells and wash by centrifugation prior to immunostaining.
NOTE: Human Fc receptors cross-react with rabbit IgG. When cells of interest express high levels of Fc receptor protein (for example, macrophage/monocyte lineages), pre-incubate live cells with human Fc block prior to immunostaining with rabbit antibodies.
NOTE: Optimal centrifugation conditions will vary depending upon cell type and reagent volume. Generally, 150-300g for 1-5 minutes will be sufficient to pellet the cells.
posted June 2017
revised January 2022
Protocol Id: 1504
Mouse
This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Killer cell lectin-like receptor G1 (KLRG1), also known as mast cell function associated antigen (MAFA), is an inhibitory type II transmembrane lectin-like receptor (1,2). This glycoprotein is expressed on a subset of natural killer (NK) and T cells (3,4). KLRG1 is considered a marker of T cell senescence (5,6). Studies have shown that KLRG1 is upregulated by MHC Class I molecule expression and these interactions can be mediated by lectin-like class I-specific Ly49 inhibitory receptors (1,3). KLRG1 triggering interferes with the activating TCR pathway and inhibits cytotoxicity (2). Specifically, KLRG1 binds to cadherin family ligands (E-, N-, and R-), inhibiting NK cell cytotoxicity (7). The effect that KLRG1 has on NK cell differentiation, maturation, homeostasis, and activation, make it an important target when studying innate immune regulation and chronic viral infections (8).
The 2F1 antibody is widely used to detect KLRG1 on mouse cells (1).
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