MHC Class II (I-E alpha) (F8N7J) Rabbit Monoclonal Antibody (Alexa Fluor® 647 Conjugate) #49980
- IHC
Product Specifications
| REACTIVITY | M |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- M-Mouse
Product Information
Product Description
Fluorescent Properties
- ← Excitation: 650 nm ← Emission: 668 nm
Product Usage Information
| Application | Dilution |
|---|---|
| Immunohistochemistry (Paraffin) | 1:50 - 1:200 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
In mice, MHC class II molecules are encoded by the H2 gene complex, which includes two main isotypes: I-A and I-E. I-A and I-E are each composed of an α and β chain. The expression and polymorphism of these molecules vary between mouse strains, influencing immune responses, disease susceptibility, and compatibility in transplantation models (4). MHC Class II (I-Eα) pairs with the I-Eβ chain to form a heterodimer that is essential for antigen presentation to CD4+ T cells. MHC Class II (I-Eα) expression is strain-dependent, making it a valuable marker in immunology and transplantation studies (5,6).
- Ting, J.P. and Trowsdale, J. (2002) Cell 109 Suppl, S21-33.
- Cresswell, P. (1994) Annu Rev Immunol 12, 259-93.
- Karp, D.R. et al. (1990) J Exp Med 171, 615-28.
- Lafuse, W.P. (1991) Crit Rev Immunol 11, 167-94.
- Janitz, M. et al. (1998) Immunogenetics 48, 266-72.
- Ibnou-Zekri, N. et al. (1997) Proc Natl Acad Sci USA 94, 14654-9.
Alternate Names
AI323765; E-alpha-f; H-2 class II histocompatibility antigen, E-D alpha chain; H-2 class II histocompatibility antigen, E-K alpha chain; H-2 class II histocompatibility antigen, E-U alpha chain; H-2 class II, E-K alpha; H-2E; H-2Ea; H2-Ea; H2-Ea-ps; H2-Ea(K); H2-IE-alpha; HA22; histocompatibility 2, class II antigen E alpha; histocompatibility 2, class II antigen E alpha, pseudogene; I-E; I-E alpha MHC class II; I-Ealpha; Ia-3; Ia3; MHC class II alpha chain; MHC class II antigen E alpha; MHC class II antigen IEk-alpha; MHC class II H2-E alpha chain; MHC class II protein; MHC-H2-Ea
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.