|100 ug/ml||Endogenous||Mouse IgG1|
Confocal immunofluorescent analysis of HeLa cells using β-Catenin (L54E2) Mouse mAb (Alexa Fluor® 555 Conjugate) #5612 (red, left) compared to concentration matched Mouse (G3A1) mAb IgG1 Isotype Control (Alexa Fluor® 555 Conjugate) (right). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).Learn more about how we get our images
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
NOTE: Cells should be grown, treated, fixed and stained directly in multi-well plates, chamber slides or on coverslips.
Aspirate liquid, then cover cells to a depth of 2–3 mm with 4% formaldehyde diluted in 1X PBS.
NOTE: Formaldehyde is toxic, use only in a fume hood.
NOTE: All subsequent incubations should be carried out at room temperature unless otherwise noted in a humid light-tight box or covered dish/plate to prevent drying and fluorochrome fading.
posted November 2006
revised November 2013
Protocol Id: 182
Note: This control antibody must be diluted to the same concentration (not dilution) as the specific antibody in analysis. See Directions on Use.Directions for Use: Important! This control antibody must be diluted to the same concentration (not dilution) as the specific antibody used for analysis. Higher background fluorescence may result if excessive amounts of mouse IgG isotype control are used. Do not use this antibody at 1:50 dilution in the same way as recommended for other CST Alexa Fluor® conjugated antibodies.
Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Mouse (G3A1) mAb IgG1 Isotype Control (Alexa Fluor® 555 Conjugate) is not directed against any known antigen. It functions as an isotype control for mouse IgG1 monoclonal antibodies.
This Cell Signaling Technology (CST) antibody is conjugated to Alexa Fluor® 555 fluorescent dye under optimal conditions and tested in-house for direct immunofluorescent analysis in human cells.
Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. DRAQ5 is a registered trademark of Biostatus Limited. The Alexa Fluor dye antibody conjugates in this product are sold under license from Life Technologies Corporation for research use only, except for use in combination with DNA microarrays. The Alexa Fluor® dyes (except for Alexa Fluor® 430 dye) are covered by pending and issued patents. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
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