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8527
Mouse (G3A1) mAb IgG1 Isotype Control (Alexa Fluor® 594 Conjugate)
Antibody Conjugates

Mouse (G3A1) mAb IgG1 Isotype Control (Alexa Fluor® 594 Conjugate) #8527

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Confocal immunofluorescent analysis of HT-29 cells using EpCAM (VU1D9) Mouse mAb (Alexa Fluor® 594 Conjugate) #7319 (left) compared to Mouse (G3A1) mAb IgG1 Isotype Control (Alexa Fluor® 594 Conjugate) (right) at matched concentration. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Flow cytometric analysis of unpermeablized HT-29 cells using EpCAM (VU1D9) Mouse mAb (Alexa Fluor® 594 Conjugate) (solid line) compared to concentration-matched Mouse (G3A1) mAb IgG1 Isotype Control (Alexa Fluor® 594 Conjugate) #8527 (dashed line).

To Purchase # 8527S
Product # Size Price
8527S
100 µl $ 115

Supporting Data

Concentration 100 ug/ml
SENSITIVITY Endogenous
Isotype Mouse IgG1, kappa

Application Key:

  • W-Western
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 594 fluorescent dye and tested in-house for direct immunofluorescent analysis in human cells.

Product Usage Information

Note: This control antibody must be diluted to the same concentration (not dilution) as the specific antibody in analysis. See Directions on Use.

Directions for Use: Important! This control antibody must be diluted to the same concentration (not dilution) as the specific antibody used for analysis. Higher background fluorescence may result if excessive amounts of mouse IgG isotype control are used. Do not use this antibody at 1:50 dilution in the same way as recommended for other CST Alexa Fluor® conjugated antibodies.

Storage:

Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Specificity / Sensitivity

Mouse (G3A1) mAb IgG1 Isotype Control (Alexa Fluor® 594 Conjugate) is not directed against any known antigen. It functions as an isotype control for mouse IgG1 monoclonal antibodies.

Background

Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
DRAQ5 is a registered trademark of Biostatus Limited.
The Alexa Fluor dye antibody conjugates in this product are sold under license from Life Technologies Corporation for research use only, except for use in combination with DNA microarrays. The Alexa Fluor® dyes (except for Alexa Fluor® 430 dye) are covered by pending and issued patents. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.