Confocal immunofluorescent analysis of HT-29 cells using EpCAM (VU1D9) Mouse mAb (Alexa Fluor® 594 Conjugate) #7319 (left) compared to Mouse (G3A1) mAb IgG1 Isotype Control (Alexa Fluor® 594 Conjugate) (right) at matched concentration. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Flow cytometric analysis of unpermeablized HT-29 cells using EpCAM (VU1D9) Mouse mAb (Alexa Fluor® 594 Conjugate) (solid line) compared to concentration-matched Mouse (G3A1) mAb IgG1 Isotype Control (Alexa Fluor® 594 Conjugate) #8527 (dashed line).
|Isotype||Mouse IgG1, kappa|
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 594 fluorescent dye and tested in-house for direct immunofluorescent analysis in human cells.
Note: This control antibody must be diluted to the same concentration (not dilution) as the specific antibody in analysis. See Directions on Use.Directions for Use: Important! This control antibody must be diluted to the same concentration (not dilution) as the specific antibody used for analysis. Higher background fluorescence may result if excessive amounts of mouse IgG isotype control are used. Do not use this antibody at 1:50 dilution in the same way as recommended for other CST Alexa Fluor® conjugated antibodies.
Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Mouse (G3A1) mAb IgG1 Isotype Control (Alexa Fluor® 594 Conjugate) is not directed against any known antigen. It functions as an isotype control for mouse IgG1 monoclonal antibodies.
Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
DRAQ5 is a registered trademark of Biostatus Limited.
The Alexa Fluor dye antibody conjugates in this product are sold under license from Life Technologies Corporation for research use only, except for use in combination with DNA microarrays. The Alexa Fluor® dyes (except for Alexa Fluor® 430 dye) are covered by pending and issued patents. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.