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4379
p44/42 MAPK (Erk1/2) (3A7) Mouse mAb (Alexa Fluor® 647 Conjugate)
Antibody Conjugates

p44/42 MAPK (Erk1/2) (3A7) Mouse mAb (Alexa Fluor® 647 Conjugate) #4379

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# Product Name Application Reactivity
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H M R Hm Mk Mi Dm Z B Dg Pg Ce
Flow Titer

Flow cytometric analysis of Jurkat cells, U0126-treated (left) or PMA-treated (right), using p44/42 MAPK (3A7) Mouse mAb (Alexa Fluor® 647 Conjugate) and Phospho-p44/42 MAPK (T202/Y204) (E10) Mouse mAb (Alexa Fluor® 488 Conjugate) #4374.

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Storage:

Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

p44/42 MAP Kinase (3A7) Mouse mAb detects endogenous levels of total p42 MAP kinase (Erk2) protein. The antibody also recognizes p44 MAP kinase (Erk1) in some cell types, although with lower affinity. It does not cross-react with either JNK/SAPK or p38 MAP kinase.

Species Reactivity:

Human, Mouse, Rat, Hamster, Monkey, Mink, Zebrafish, Bovine, Pig

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of rat p42 MAP kinase.

The antibody was conjugated with Alexa Fluor® 647 under optimum conditions with a F/P ratio of 2-6. The Alexa Fluor® 647 dye is maximally excited by red light (e.g. 633 nm He-Ne laser). Antibody conjugates of the Alexa Fluor® 647 dye produce bright far-red-fluorescence emission, with a peak at 665 nm.

Cell Signaling Technology Antibody conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct Flow Cytometric analysis of human cells.

*The unconjugated antibody #9107 reacts with, among others, human, mouse, rat, and hamster p42 MAPK. CST expects that p42 MAPK (3A7) Mouse mAb (Alexa Fluor® 647 Conjugate) will also recognize p42 MAPK in these species.

Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs, such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines (1-3), and research investigators consider it an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family, as well as Mos and Tpl2/COT. MEK1 and MEK2 are the primary MAPKKs in this pathway (5,6). MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK (7) and the transcription factor Elk-1 (8,9). p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs (10), along with MEK inhibitors, such as U0126 and PD98059.

  1. Roux, P.P. and Blenis, J. (2004) Microbiol Mol Biol Rev 68, 320-44.
  2. Baccarini, M. (2005) FEBS Lett 579, 3271-7.
  3. Meloche, S. and Pouysségur, J. (2007) Oncogene 26, 3227-39.
  4. Roberts, P.J. and Der, C.J. (2007) Oncogene 26, 3291-310.
  5. Rubinfeld, H. and Seger, R. (2005) Mol Biotechnol 31, 151-74.
  6. Murphy, L.O. and Blenis, J. (2006) Trends Biochem Sci 31, 268-75.
  7. Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505.
  8. Marais, R. et al. (1993) Cell 73, 381-93.
  9. Kortenjann, M. et al. (1994) Mol Cell Biol 14, 4815-24.
  10. Owens, D.M. and Keyse, S.M. (2007) Oncogene 26, 3203-13.
Entrez-Gene Id
5595 , 5594
Swiss-Prot Acc.
P27361 , P28482
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
The Alexa Fluor dye antibody conjugates in this product are sold under license from Life Technologies Corporation for research use only, except for use in combination with DNA microarrays. The Alexa Fluor® dyes (except for Alexa Fluor® 430 dye) are covered by pending and issued patents. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

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